Latrophilin 3/LPHN3 Antibody (801518)

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Latrophilin 3/LPHN3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human brain (hypothalamus) tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Latrophilin 3/LPHN3 at approximately 109 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 801518
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Latrophilin 3/LPHN3 Antibody (801518) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Latrophilin 3/LPHN3
  Met1-Arg813
  Accession # Q9HAR2
• Specificity: Detects human Latrophilin 3/LPHN3 in direct ELISAs and human, mouse, and rat Latrophilin 3/LPHN3 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LPHN3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Latrophilin 3/LPHN3 Antibody (801518)

• CIRL3
• Latrophilin 3
• latrophilin homolog 3 (cow)
• LEC3
• Lectomedin-3
• LPHN3

Background

LPHN3 (Latrophilin 3; also CIRL3, CL3 and LEC3) is a (presumably) 220 kDa member of the LN-TM7 subfamily, GPCR 2 family of molecules. It appears to have a restricted expression pattern, being limited to brain and adrenal gland. Although it is related to the black widow toxin receptor CIRL1, it does not serve as a toxin receptor. Mature human Latrophilin 3 is a 7-TM glycoprotein that is 1428 amino acids (aa) in length. Post-translational processing cleaves the molecule into a 120 kDa ECD (aa 20-841) and a noncovalently-associated 85 kDa 7-TM C-terminus (aa 842-1447). The ECD is modular and contains a SUEL-like lectin domain (aa 35-124), an Olf region (aa 134-393) and GPS domain (aa 802-853). There are multiple splice events which, in the ECD, include a two aa substitution for aa 127-131, a 13 aa insertion after Lys623, and a 39 aa substitution for aa 668-1447. Over aa 20-832, human Latrophilin 3 shares 98% aa identity with mouse Latrophilin 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Latrophilin 3/LPHN3 Antibody (MAB5916)(1)

Publication using MAB5916

• DM Virga, S Hamilton, B Osei, A Morgan, E Zamponi, NJ Park, VL Hewitt, D Zhang, KC Gonzalez, E Bloss, F Polleux, TL Lewis Activity-dependent subcellular compartmentalization of dendritic mitochondria structure in CA1 pyramidal neurons bioRxiv : the preprint server for biology, 2023-03-26;0(0):. 2023-03-26 [PMID: 36993655] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: LPHN3
• Uniprot:
  • Q9HAR2(Human)