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Lass5 Antibody

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Lass5 Antibody Summary

Immunogen
Peptide with sequence C-RGKVSKDDRSDVES corresponding to internal region according to NP_982288.1.
Epitope
internal region
Specificity
This antibbody is expected to cross-react with the highly similar LASS6.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CERS5
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in lysates of HEK293, HeLa and HepG2 after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.8 kDa band according to NP_671723.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-00140.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Lass5 Antibody

  • CerS5
  • FLJ25304
  • LAG1 homolog, ceramide synthase 5
  • LAG1 longevity assurance homolog 5 (S. cerevisiae)
  • LAG1 longevity assurance homolog 5
  • MGC45411
  • Trh4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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LASS6 Antibody
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Western Blot: LASS2 Antibody (1A6) [H00029956-M01A] - S2 monoclonal antibody (M01), clone 1A6. Western Blot analysis of LASS2 expression in NIH/3T3.
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Western Blot: LASS4 Antibody (7D5) [H00079603-M03] - Analysis of LASS4 expression in transfected 293T cell line by LASS4 monoclonal antibody (M03), clone 7D5. Lane 1: LASS4 transfected lysatE (46.4 KDa). Lane 2: Non-transfected lysate.
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Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.
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Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).
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CCL4/MIP-1 beta
[HRP]
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Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6. Analysis of SPHK1 expression in K-562.
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NUP62 Antibody
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LC3B Antibody
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Knockout Validated: LC3B Antibody [NB100-2220] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
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Bax Antibody (6A7)
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Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review
Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a varified end user.
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Bioinformatics

Gene Symbol CERS5
Entrez
Uniprot