Lass5 Antibody

Product Discontinued

Lass5 Antibody Summary

• Immunogen: Peptide with sequence C-RGKVSKDDRSDVES corresponding to internal region according to NP_982288.1.
• Epitope: internal region
• Specificity: This antibbody is expected to cross-react with the highly similar LASS6.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CERS5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments gave an approx. 60 kDa band in lysates of HEK293, HeLa and HepG2 after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.8 kDa band according to NP_671723.1. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Lass5 Antibody

• CerS5
• FLJ25304
• LAG1 homolog, ceramide synthase 5
• LAG1 longevity assurance homolog 5 (S. cerevisiae)
• LAG1 longevity assurance homolog 5
• MGC45411
• Trh4

Background

Lass5 may be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed incells is involved in the production of sphingolipids containingmainlyone fatty acid donnor (N-linked palmitoyl-(C16) ceramide) in a fumonisin B1-in

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lass5 Antibody (NBP1-00140)(1)

Publication using NBP1-00140

• Min J, Mesika A, Sivaguru M et al. (Dihydro)ceramide synthase 1 regulated sensitivity to cisplatin is associated with the activation of p38 mitogen-activated protein kinase and is abrogated by sphingosine kinase 1. Mol Cancer Res 2007-08-01 [PMID: 17699106]

Bioinformatics

• Gene Symbol: CERS5
• Entrez:
  • 91012(Human)
  • 71949(Mouse)
  • 366984(Rat)
• Uniprot:
  • Q8N5B7(Human)