LASP1 Antibody

Western Blot: LASP1 Antibody [NB100-77338] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LASP1 Antibody Summary

• Description: Novus Biologicals Goat LASP1 Antibody (NB100-77338) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-LASP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PERRDSQDGSSYR corresponding to internal region according to NP_006139.1.
• Epitope: PERRDSQDGSSYR
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LASP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.03-0.1 ug/ml
• Application Notes: Western blot: Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30;207(2):171-5; PMID: 9511759).
  IHC: Paraffin embedded Human Colon and Small Intestine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LASP1 Antibody

• LASP-1
• LIM and SH3 domain protein 1
• LIM and SH3 protein 1
• Metastatic lymph node gene 50 protein
• MLN 50
• MLN50Lasp-1

Background

This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LASP1 Antibody (NB100-77338)(1)

Publication using NB100-77338

• Grunewald TG, Kammerer U, Schulze E et al. Silencing of LASP-1 influences zyxin localization, inhibits proliferation and reduces migration in breast cancer cells. Exp Cell Res 2006-04-15 [PMID: 16430883]

Bioinformatics

• Gene Symbol: LASP1
• Entrez:
  • 3927(Human)
• Uniprot:
  • NP_006139.1(Human)