LASP1 Antibody

Images

 
Western Blot: LASP1 Antibody [NB100-77338] - (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: LASP1 Antibody [NB100-77338] - (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

LASP1 Antibody Summary

Immunogen
Peptide with sequence C-PERRDSQDGSSYR corresponding to internal region according to NP_006139.1.
Epitope
PERRDSQDGSSYR
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LASP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03-0.1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx 36kDa band observed in Human Kidney lysates (calculated MW of 29.7kDa according to NP_006139.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreiber et al, Gene. 1998 Jan 30;207(2):171-5; PMID: 9511759).
IHC: Paraffin embedded Human Colon and Small Intestine.
Publications
Read Publication using NB100-77338.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LASP1 Antibody

  • LASP-1
  • LIM and SH3 domain protein 1
  • LIM and SH3 protein 1
  • Metastatic lymph node gene 50 protein
  • MLN 50
  • MLN50Lasp-1

Background

This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LASP1 Antibody (NB100-77338)(1)

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Product General Protocols

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Bioinformatics

Gene Symbol LASP1
Entrez
Uniprot