LAR/PTPRF Antibody - BSA Free

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. (anti-PTPRF)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

LAR/PTPRF Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken LAR/PTPRF Antibody - BSA Free (NB100-75290) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was a sequence from 1151a.a. to 1225a.a. from LAR(a 1897a.a. long protein)
• Localization: Cytoplasm. Colocalizes with PTPRF at the ends of focal adhesions most proximal to the cell nucleus.
• Specificity: LAR
• Clonality: Polyclonal
• Host: Chicken
• Gene: PTPRF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:200 - 1:500
• Application Notes: Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

Reactivity Notes

This antibody is reactive against Human, mouse, rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for LAR/PTPRF Antibody - BSA Free

• LAR
• LCA-homolog
• protein tyrosine phosphatase, receptor type, F
• PTPRF
• receptor type, F polypeptide
• receptor-linked protein-tyrosine phosphatase LAR
• receptor-type tyrosine-protein phosphatase F

Background

Leukocyte common antigen-related (LAR) family receptor protein tyrosine phosphatases (LAR-RPTP) bind to liprin-alpha (SYD2) and are implicated in axon guidance. LAR is also involved in the regulation of epithelial cell-cell contacts and in the control of beta-catenin signaling. Increased LAR expression is found in the insulin-responsive tissue of obese insulin-resistant individuals and may contribute to the pathogenesis of insulin resistance. LAR is a receptor-type PTP and possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains. The extracellular region contains three Ig-like domains, and nine domains similar to that of neural-cell adhesion molecule. Several isoforms of LAR have been reported. Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase).. The first PTPase domain has enzymatic activity. while the second one seems to affect the substrate specificity of the first one.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRF
• Entrez:
  • 5792(Human)
  • 19268(Mouse)
  • 360406(Rat)
• Uniprot:
  • P10586(Human)