LAR/PTPRF Antibody [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: CoraFluor 1

LAR/PTPRF Antibody [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LAR
  Ala27-Glu1251
  Accession # NP_569707
• Specificity: Detects human, mouse, and rat LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular (E) domain. The antibody does not cross react with PTPR sigma .
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPRF
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for LAR/PTPRF Antibody [CoraFluor™ 1]

• LAR
• LCA-homolog
• protein tyrosine phosphatase, receptor type, F
• PTPRF
• receptor type, F polypeptide
• receptor-linked protein-tyrosine phosphatase LAR
• receptor-type tyrosine-protein phosphatase F

Background

PTPRF, also known as Receptor-type tyrosine-protein phosphatase F, is a 213kDa 1907 amino acid protein with a shorter 212kDa 1898 isoform. PTPRF is a member of the protein tyrosine phosphatase (PTP) family and functions as a possible cell adhesion receptor. Research is being conducted on PTPRF in relation to melanoma, carcinoma, retinitis, thyroid carcinoma, ureterocele, lung cancer, breast cancer and obesity. PTPRF is linked to the cell adhesion and insulin signaling pathways where it interacts with CAV1, PRPF40A, SKIL, ZNF512B and JUP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRF