LAR/PTPRF Antibody (384727) [Unconjugated]

Western blot shows lysates of CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human LAR Monoclonal Antibody (Catalog # MAB3004) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LAR at approximately 139 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 384727
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

LAR/PTPRF Antibody (384727) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LAR
  Ala27-Glu1251
  Accession # NP_569707
• Specificity: Detects endogenous human LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular domain (ECD). It does not cross-react with recombinant human PTPRM, PTPRK, DEP1, or PTPR gamma .
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: PTPRF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAR/PTPRF Antibody (384727) [Unconjugated]

• LAR
• LCA-homolog
• protein tyrosine phosphatase, receptor type, F
• PTPRF
• receptor type, F polypeptide
• receptor-linked protein-tyrosine phosphatase LAR
• receptor-type tyrosine-protein phosphatase F

Background

Leukocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phosphatase, Receptor-type F (PTPRF), is an integral membrane protein with anon-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRF
• Uniprot:
  • NP_569707()