Laminin beta 3 Antibody (CL3353) - BSA Free

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221.

Western Blot: Laminin beta 3 Antibody (CL3353) [NBP2-46622] - Analysis in human lung tissue

Staining of human skin shows moderate positivity in the basement membrane of the basal layer of epidermal cells.

Staining of rat kidney shows strong positivity in basement membrane in cells in glomeruli.

Staining of rat lung shows moderate to strong positivity in basement membrane of pneumocytes.

Staining of rat rectum shows moderate positivity in basement membrane of glandular cells, as well as strong positivity in endothelial cells.

Staining of rat skin shows moderate to strong positivity in basement membrane basal epidermal cells.

Staining of human pancreas shows no positivity in glandular cells as expected.

Staining of human prostate shows moderate to strong positivity in basement membrane of glandular cells.

Staining of human fallopian tube shows moderate to strong positivity in basement membrane of glandular cells.

Staining of A-431 cells, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL3353
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Laminin beta 3 Antibody (CL3353) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q13751, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  Laminin beta 3 Recombinant Protein Antigen (NBP2-46622PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Laminin beta 3 Antibody (CL3353) - BSA Free

• BM600-125kDa
• Epiligrin subunit bata
• FLJ99565
• Kalinin B1 chain
• Kalinin subunit beta
• kalinin-140kDa
• LAM5
• Laminin 5
• Laminin B1k chain
• laminin S B3 chain
• laminin subunit beta-3
• laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD))
• laminin, beta 3
• Laminin-5 subunit beta
• LAMNB1
• Nicein subunit beta
• nicein-125kDa

Background

The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin beta 3 Antibody (NBP2-46622)(2)

Publications using NBP2-46622

• Shiju TM, Sampaio LP, Hilgert GSL, Wilson SE Corneal epithelial basement membrane assembly is mediated by epithelial cells in coordination with corneal fibroblasts during wound healing Molecular vision 2023-05-20 [PMID: 37287640] (IHC-Fr, Rabbit)
• de Oliveira RC, Sampaio LP, Shiju TM et al. Epithelial Basement Membrane Regeneration After PRK-Induced Epithelial-Stromal Injury in Rabbits: Fibrotic Versus Non-fibrotic Corneal Healing Journal of refractive surgery (Thorofare, N.J. : 1995) 2022-01-01 [PMID: 35020537]

Review for Laminin beta 3 Antibody (NBP2-46622) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rabbit.

reviewed by: Apu Saikia ICC Rabbit 05/04/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Rabbit tissue
• Species: Rabbit
• Lot: 02999

Comments

• Comments: 1 hour incubation at 1:500 dilution worked very well for immunohistochemistry

Bioinformatics

• Gene Symbol: LAMB3
• Entrez:
  • 3914(Human)
• Uniprot:
  • Q13751(Human)