Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

Immunohistochemistry-Paraffin: Laminin alpha 2 Antibody (2D4) [H00003908-M01] - Analysis of monoclonal antibody to LAMA2 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 1 ug/ml.

Sandwich ELISA: Laminin alpha 2 Antibody (2D4) [H00003908-M01] - Detection limit for recombinant GST tagged LAMA2 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Laminin alpha 2 Antibody (2D4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: LAMA2 (NP_000417, 3013 a.a. ~ 3122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DAGVPGHLCDGQWHKVTANKIKHRIELTVDGNQVEAQSPNPASTSADTNDPVFVGGFPDDLKQFGLTTSIPFRGCIRSLKLTKGTGKPLEVNFAKALELRGVQPVSCPAN
• Localization: Extracellular
• Specificity: LAMA2 - laminin, alpha 2 (merosin, congenital muscular dystrophy)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMA2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 27390862).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Laminin alpha 2 Antibody (2D4) - Azide and BSA Free

• LAMA2
• Laminin alpha 2
• Laminin M chain
• laminin subunit alpha-2
• laminin, alpha 2
• Laminin-12 subunit alpha
• Laminin-2 subunit alpha
• LAMM
• LAMMLaminin-4 subunit alpha
• Merosin heavy chain
• Merosin

Background

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Laminin alpha 2 Antibody (H00003908-M01)(7)

Publications using H00003908-M01

• Amani V, Riemondy KA, Fu R et al. Integration of single-nuclei RNA-sequencing, spatial transcriptomics and histochemistry defines the complex microenvironment of NF1-associated plexiform neurofibromas Acta Neuropathol Commun 2023-09-28 [PMID: 37770931]
• Graziella S, Regia L, Taciani M et al. A coordinated approach for the assessment of molecular subgroups in pediatric ependymomas using low-cost methods. J Mol Med (Berl). 2021-04-26 [PMID: 33903940]
• Sasaki A, Hirato J, Hirose T et al. Review of ependymomas: assessment of consensus in pathological diagnosis and correlations with genetic profiles and outcome. Brain Tumor Pathol. 2019-03-30 [PMID: 30929114]
• Araki A, Chocholous M, Gojo J et al. Chromosome 1q gain and tenascin-C expression are candidate markers to define different risk groups in pediatric posterior fossa ependymoma. Acta Neuropathol Commun 2016-08-22 [PMID: 27550150]
• Zakrzewska M, Fendler W, Zakrzewski K et al. Altered MicroRNA Expression Is Associated with Tumor Grade, Molecular Background and Outcome in Childhood Infratentorial Ependymoma. PLoS One 2016-07-08 [PMID: 27390862] (IF/IHC)
• Dong H, Ding H, Young K et al. Advanced Intimal Hyperplasia Without Luminal Narrowing of Leptomeningeal Arteries in CADASIL. Stroke. 2013-03-12 [PMID: 23482599]
• Lemons JM, Feng XJ, Bennett BD et al. Quiescent fibroblasts exhibit high metabolic activity. PLoS Biol 2010-10-01 [PMID: 21049082]

Bioinformatics

• Gene Symbol: LAMA2
• Entrez:
  • 3908(Human)
• Uniprot:
  • NP_000417(Human)