Lamin B1 Antibody

Product Discontinued

Lamin B1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human Lamin B1.
• Marker: Nuclear Envelope Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LMNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Lamin B1 (L75) pAb in extracts from COLO cells.
  Immunohistochemistry (IHC) analyzes of Lamin B1 (L75) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lamin B1 Antibody

• ADLD
• Lamin B1
• lamin-B1
• LMN
• LMN2
• LMNB
• LMNB1
• MGC111419

Background

An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lamin B1 Antibody (NBP1-19804)(4)

Publications using NBP1-19804

• Zhang S, Chen Y, Dong K et al. BESST: a novel LncRNA knockout strategy with less genome perturbance Nucleic acids research 2023-03-20 [PMID: 36938886] (WB, Human)
• Buffolo F, Petrosino V, Albini M et al. Neuroinflammation induces synaptic scaling through IL-1 beta-mediated activation of the transcriptional repressor REST/NRSF Cell death & disease 2021-02-15 [PMID: 33589593] (WB, Mouse)
• Fernandez Larrosa PN, Ruiz Grecco M, Mengual Gomez D et al. RAC3 more than a nuclear receptor coactivator: a key inhibitor of senescence that is downregulated in aging. Cell Death Dis 2015-01-01 [PMID: 26469953] (WB)
• Sun B, Kawahara M, Ehata S et al. AAG8 promotes carcinogenesis by activating STAT3. Cell Signal 2014-04-12 [PMID: 24726897] (WB, Human)
  
  Details:
  Using the HRP conjugated version of NB500-201, catalog number NB500-201H.

FAQs for Lamin B1 Antibody (NBP1-19804). (Showing 1 - 1 of 1 FAQs).

1. I'm looking for human lamin antibodies that might cross react with Drosophila lamins. I've found a few that I'm interested in, one of which comes in a smaller sample size. The other two do not have a smaller size ordering option. I was wondering if it would be possible to get these other antibodies in a smaller size as well, so we can test reactivity with Drosophila lamins before ordering the full-sized product?
   • We have a wide range of antibodies to human Lamin. Unfortunately none of these has yet been tested against Drosophila samples, however you may be interested in our Innovator's Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. We are unable to offer free samples of our antibodies but, as you rightly point out, some are available as smaller, sample size aliquots. If a sample size is not listed for a product I am afraid we are unable to offer a smaller aliquot than those already listed.

Bioinformatics

• Gene Symbol: LMNB1
• Entrez:
  • 4001(Human)
  • 16906(Mouse)
  • 116685(Rat)
• Uniprot:
  • P20700()