LAG-3 Antibody



Product Details

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LAG-3 Antibody Summary

Synthetic peptide, C-QPRVQLDERGRQRGD, representing the internal region of the human sequence according to NP_002277.3
Immunogen affinity purified
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Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-saline [ pH7.3] with 0.5% BSA
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments in Human Lymph Node, Spleen and Tonsil lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Theoretical MW
57 kDa

Reactivity Notes



Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at for more details.


Lymphocyte-activation protein 3 (LAG-3), also known as CD223, belongs to the immunoglobulin superfamily and contains 4 extracellular Ig-like domains. LAG-3 binds to HLA class-II antigens and is involved in lymphocyte activation. It is found on the cell surface of activated NK and T-lymphocytes. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG-3 to CD4.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAG-3 Antibody (NB100-57857) (0)

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FAQs for LAG-3 Antibody (NB100-57857). (Showing 1 - 4 of 4 FAQ).

  1. What is the concentration of your biotin conjugated LAG-3 antibody?
    • We actuall do not determine the concentration of our conjugated antibodies but they are usuall 80-100% the concentration of the base product, so the concentration should be at least 0.8 mg/ml.
  2. Can this LAG-3 antibody be used in ELISA?
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  3. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.
  4. Is this LAG-3 antibody offered in a smaller size that is less expensive?
    • We do not offer a sample size for this particular LAG 3 antibody but we do offer another, AF3329, in a 25 ug sample size.

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Contact Information

Product PDFs


Gene Symbol LAG3

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