LAG-3 Antibody (C9B7W)

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Flow Cytometry: LAG-3 Antibody (C9B7W) [NB100-63601] - Staining of CD223 transfected cells with RAT ANTI MOUSE CD223

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
C9B7W
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

LAG-3 Antibody (C9B7W) Summary

Immunogen
Murine CD223 Ig fusion protein
Specificity
NB100-63601 recognizes murine lymphocyte activation gene-3 (LAG-3), a 70kD activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognizes an epitope within the D2 domain of CD223. The antibody is reported to block the in vitro function of murine LAG-3 but studies suggest that the antibody does not block binding of LAG-3 to MHC class II ^(1).
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
LAG3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:200
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for LAG-3 Antibody (C9B7W)

  • CD223 antigen
  • CD223
  • FDC
  • LAG3
  • LAG-3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Protein FDC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for LAG-3 Antibody (NB100-63601). (Showing 1 - 4 of 4 FAQ).

  1. What is the concentration of your biotin conjugated LAG-3 antibody?
    • We actuall do not determine the concentration of our conjugated antibodies but they are usuall 80-100% the concentration of the base product, so the concentration should be at least 0.8 mg/ml.
  2. Can this LAG-3 antibody be used in ELISA?
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  3. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.
  4. Is this LAG-3 antibody offered in a smaller size that is less expensive?
    • We do not offer a sample size for this particular LAG 3 antibody but we do offer another, AF3329, in a 25 ug sample size.

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Bioinformatics

Gene Symbol LAG3
Entrez
Uniprot