LAG-3 Antibody (17B4)

Images

 
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using LAG-3 (human), mAb (17B4).
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. LAG-3 staining in resting and PHA activated lymphocytes. Image submitted by a verified customer review.
Western Blot: LAG-3 Antibody (17B4) [NBP1-97657] - Lane 1: MW marker. Lane 2: LAG-3 (human):Fc (human).
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Staining of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with LAG-3 ...read more
Immunohistochemistry-Paraffin: LAG-3 Antibody (17B4) [NBP1-97657] - LAG-3 in human tonsil tissue sample. Image submitted by a verified customer review.
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. LAG-3 expression on activated human peripheral blood mononuclear cells (PBMC) detected with LAG-3 (human), mAb (17B4) ...read more
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Method: LAG-3 (human), mAb (17B4) (FITC) (10 ug/mL) (is preincubated with a specific peptide epitope (208b) or a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
17B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

LAG-3 Antibody (17B4) Summary

Immunogen
Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).
Specificity
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LAG3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/mL
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 10 ug/mL
Control
LAG-3 Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP1-97657 in the following applications:

Publications
Read Publications using
NBP1-97657 in the following applications:

  • 2 publications
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 10% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for LAG-3 Antibody (17B4)

  • 17b4 lag3
  • 17b4 neutralizing
  • 17b4
  • anti human lag3
  • Anti-LAG-3 Antibody
  • CD223 Antibody
  • CD223 Antigen Antibody
  • CD223 antigen
  • CD223
  • FDC
  • Human LAG3 Antibody
  • Human LAG-3 Antibody
  • LAG-3 17b4
  • LAG-3 blocking
  • LAG3
  • LAG-3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Protein FDC

Background

The lymphocyte activation gene-3, also called CD223, codes for a 70 kDa type I transmembrane glycoprotein and is similar to CD4 in terms of structure and their major ligand. LAG-3 (lymphocyte activation gene-3) is part of the IgSF (immunoglobulin super family) and binds to MHC II like CD4 but with greater affinity. LAG-3 has been shown to play a major role in the negative regulation of immune responses and soluble LAG-3 may serve as a serological marker for examining Th1 responses. In addition to negatively regulating T cell expansion LAG-3 has also been shown to up regulate certain immune responses through MHC II signaling in antigen presenting cells. LAG-3 and CD49b are co-expressed on Type 1 regulatory T cells and are potential markers for identifying Tr1 cells by dual staining. LAG-3 has numerous alternative splice variants including two secreted forms. LAG-3 is primarily expressed on Treg cells, activated T cells and NK cells. This LAG-3 antibody was made to a 30 aa 'extra loop' of the first N-terminal D1 domain of LAG-3 so that it will not cross react with the closely related CD4 transmembrane protein(1). Both transmembrane and secreted forms are recognized by this LAG-3 antibody. In the same study the LAG-3 antibody was shown to detect a 70 kDa protein corresponding to the transmembrane LAG-3 protein and a 45 kDa protein secreted LAG-3 protein. Another study used this LAG-3 antibody to demonstrate the effects of blocking LAG-3 / MHC II interactions with anti LAG-3 antibody(2). Enhanced T cell expansion was observed in samples incubated with the LAG-3 antibody that prevents MHC II / LAG-3 interactions; indicating that LAG-3 is involved in regulating T-cell expansion(2). This LAG-3 antibody was used in another study that set out to demonstrate the potential of using soluble LAG-3 as a vaccine adjuvant due to its role in stimulating antigen preventing cells(3). PMID(s): 1. 1380059: LAG-3 Antibody Used as a Cellular Marker to Reveal Expression Patterns 2. 15885122: Blocking of MHC II ' LAG-3 Interactions with LAG-3 Antibody 3. 18322184: Effect of Soluble LAG-3 Peptide on DC Activation Measured with LAG-3 Antibody

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAG-3 Antibody (NBP1-97657)(14)

We have publications tested in 2 confirmed species: Human, Primate.

We have publications tested in 2 applications: Flow, IHC.


Filter By Application
Flow
(2)
IHC
(1)
All Applications
Filter By Species
Human
(4)
Primate
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-97657 Applications Species
Harper J, Gordon S, Chan C et Al. CTLA-4 and PD-1 dual blockade induces SIV reactivation without control of rebound after antiretroviral therapy interruption Nat Med. [PMID: 32284611] (Flow, Primate) Flow Primate
Pericart S, Tosolini M, Gravelle P et al. Profiling Immune Escape in Hodgkin's and Diffuse large B-Cell Lymphomas Using the Transcriptome and Immunostaining Cancers (Basel) 2018 Oct 31 [PMID: 30384489] (IHC, Human)

Details:
Citation using the FITC form of this antibody.
IHC Human
Wang J, Ti Y, Wang Y et al. LAG-3 Represents a Marker of CD4+ T Cells with Regulatory Activity in Patients with Bone Fracture. Immunol. Invest. Apr 19 2018 [PMID: 29671649] (Human) Human
Sabins NC, Chornoguz O, Leander K et al. TIM-3 Engagement Promotes Effector Memory T Cell Differentiation of Human Antigen-Specific CD8 T Cells by Activating mTORC1 J. Immunol. 2017 Nov 10 [PMID: 29127145] (Flow, Human) Flow Human
Ma QY, Huang DY, Zhang HJ et al. Function and regulation of LAG3 on CD4(+)CD25(-) T cells in non-small cell lung cancer Exp. Cell Res. 2017 Sep 19 [PMID: 28935468] (Human) Human
Casati C, Camisaschi C, Novellino L et al. Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation. J Immunol. 2008 Mar. [PMID: 18322184]
Triebel F, Hacene K, Pichon MF et al. A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors. Cancer Lett. 2006 Apr. [PMID: 15946792]
Macon-Lemaitre L, Triebel F. The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells. Immunology. 2005 Jun. [PMID: 15885122]
Di Carlo E, Cappello P, Sorrentino C et al. Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway. J Pathol. 2005 Jan. [PMID: 15586367]
Demeure CE, Wolfers J, Martin-Garcia N et al. T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts. Eur J Cancer. 2001 Sep. [PMID: 11527700]
Show All 14 Publications.

Reviews for LAG-3 Antibody (NBP1-97657) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-97657:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 LAG-3 NBP1-97657
Enlarge
reviewed by:
Anonymous
Human 03/07/2020
View

Summary

Sample Testedhuman tonsil
SpeciesHuman
LotN/A
Immunohistochemistry-Paraffin LAG-3 NBP1-97657
Enlarge
reviewed by:
Anonymous
IHC-P Human 01/23/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTonsil
SpeciesHuman
Lot02011620

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAG-3 Antibody (NBP1-97657). (Showing 1 - 4 of 4 FAQs).

  1. What is the concentration of your biotin conjugated LAG-3 antibody?
    • We actuall do not determine the concentration of our conjugated antibodies but they are usuall 80-100% the concentration of the base product, so the concentration should be at least 0.8 mg/ml.
  2. Can this LAG-3 antibody be used in ELISA?
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  3. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.
  4. Is this LAG-3 antibody offered in a smaller size that is less expensive?
    • We do not offer a sample size for this particular LAG 3 antibody but we do offer another, AF3329, in a 25 ug sample size.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97657AF350
Alexa Fluor 405 NBP1-97657AF405
Alexa Fluor 488 NBP1-97657AF488
Alexa Fluor 532 NBP1-97657AF532
Alexa Fluor 594 NBP1-97657AF594
Alexa Fluor 647 NBP1-97657AF647
Alexa Fluor 700 NBP1-97657AF700
Alexa Fluor 750 NBP1-97657AF750
Allophycocyanin NBP1-97657APC
Allophycocyanin/Cy7 NBP1-97657APCCY7
Biotin NBP1-97657B
DyLight 350 NBP1-97657UV
DyLight 405 NBP1-97657V
DyLight 488 NBP1-97657G
DyLight 550 NBP1-97657R
DyLight 594 NBP1-97657DL594
DyLight 650 NBP1-97657C
DyLight 680 NBP1-97657FR
DyLight 755 NBP1-97657IR
FITC NBP1-97665
HRP NBP1-97657H
Janelia Fluor 549 NBP1-97657JF549
Janelia Fluor 646 NBP1-97657JF646
PE NBP1-97657PE
PE/Atto594 NBP1-97657PEATT594
PE/Cy5.5 NBP1-97657PECY55
PE/Cy7 NBP1-97657PECY7
PerCP NBP1-97657PCP

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
03/07/2020
Application:
Species: Human

Anonymous
01/23/2018
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol LAG3