Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using LAG-3 (human), mAb (17B4).
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. LAG-3 staining in resting and PHA activated lymphocytes. Image submitted by a verified customer review.
Western Blot: LAG-3 Antibody (17B4) [NBP1-97657] - Lane 1: MW marker. Lane 2: LAG-3 (human):Fc (human).
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Staining of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with LAG-3 ...read more
Immunohistochemistry-Paraffin: LAG-3 Antibody (17B4) [NBP1-97657] - LAG-3 in human tonsil tissue sample. Image submitted by a verified customer review.
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. LAG-3 expression on activated human peripheral blood mononuclear cells (PBMC) detected with LAG-3 (human), mAb (17B4) ...read more
Flow Cytometry: LAG-3 Antibody (17B4) [NBP1-97657] - Analysis using the FITC conjugate of NBP1-97657. Method: LAG-3 (human), mAb (17B4) (FITC) (10 ug/mL) (is preincubated with a specific peptide epitope (208b) or a ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 10% glycerol
0.02% Sodium Azide
Protein A or G purified
Alternate Names for LAG-3 Antibody (17B4)
anti human lag3
CD223 Antigen Antibody
Human LAG3 Antibody
Human LAG-3 Antibody
lymphocyte activation gene 3 protein
lymphocyte-activation gene 3
The lymphocyte activation gene-3, also called CD223, codes for a 70 kDa type I transmembrane glycoprotein and is similar to CD4 in terms of structure and their major ligand. LAG-3 (lymphocyte activation gene-3) is part of the IgSF (immunoglobulin super family) and binds to MHC II like CD4 but with greater affinity. LAG-3 has been shown to play a major role in the negative regulation of immune responses and soluble LAG-3 may serve as a serological marker for examining Th1 responses. In addition to negatively regulating T cell expansion LAG-3 has also been shown to up regulate certain immune responses through MHC II signaling in antigen presenting cells. LAG-3 and CD49b are co-expressed on Type 1 regulatory T cells and are potential markers for identifying Tr1 cells by dual staining. LAG-3 has numerous alternative splice variants including two secreted forms. LAG-3 is primarily expressed on Treg cells, activated T cells and NK cells. This LAG-3 antibody was made to a 30 aa 'extra loop' of the first N-terminal D1 domain of LAG-3 so that it will not cross react with the closely related CD4 transmembrane protein(1). Both transmembrane and secreted forms are recognized by this LAG-3 antibody. In the same study the LAG-3 antibody was shown to detect a 70 kDa protein corresponding to the transmembrane LAG-3 protein and a 45 kDa protein secreted LAG-3 protein. Another study used this LAG-3 antibody to demonstrate the effects of blocking LAG-3 / MHC II interactions with anti LAG-3 antibody(2). Enhanced T cell expansion was observed in samples incubated with the LAG-3 antibody that prevents MHC II / LAG-3 interactions; indicating that LAG-3 is involved in regulating T-cell expansion(2). This LAG-3 antibody was used in another study that set out to demonstrate the potential of using soluble LAG-3 as a vaccine adjuvant due to its role in stimulating antigen preventing cells(3). PMID(s): 1. 1380059: LAG-3 Antibody Used as a Cellular Marker to Reveal Expression Patterns 2. 15885122: Blocking of MHC II ' LAG-3 Interactions with LAG-3 Antibody 3. 18322184: Effect of Soluble LAG-3 Peptide on DC Activation Measured with LAG-3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for LAG-3 Antibody (NBP1-97657). (Showing 1 - 4 of 4 FAQs).
What is the concentration of your biotin conjugated LAG-3 antibody?
We actuall do not determine the concentration of our conjugated antibodies but they are usuall 80-100% the concentration of the base product, so the concentration should be at least 0.8 mg/ml.
Can this LAG-3 antibody be used in ELISA?
We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.
Is this LAG-3 antibody offered in a smaller size that is less expensive?
We do not offer a sample size for this particular LAG 3 antibody but we do offer another, AF3329, in a 25 ug sample size.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.