Lactoferrin Antibody (1C6cc) [DyLight 405]

• Reactivity: Hu
• Applications: WB, ELISA, IHC, CyTOF-ready
• Clone: 1C6cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 405

Lactoferrin Antibody (1C6cc) [DyLight 405] Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified lactoferrin from human milk.
• Localization: Secreted
• Specificity: Human lactoferrin. No cross-reactivity with human transferrin.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Lactoferrin Antibody (1C6cc) [DyLight 405]

• EC 3.4.21
• EC 3.4.21.-
• GIG12
• growth-inhibiting protein 12
• HEL110
• HLF2
• Kaliocin-1
• Lactoferricin
• Lactoferrin
• Lactoferroxin
• Lactotransferrin
• LF
• LTF
• neutrophil lactoferrin
• Talalactoferrin

Background

Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LTF
• Entrez:
  • 4057(Human)
• Uniprot:
  • P02788(Human)