Kv4.2 Antibody (5B11B9) - BSA Free

Western Blot: Kv4.2 Antibody (5B11B9) [NBP2-52497] - Western blot analysis using KCND2 mAb against human KCND2 (AA: 27-184) recombinant protein. (Expected MW is 42.3 kDa)

Flow Cytometry: Kv4.2 Antibody (5B11B9) [NBP2-52497] - Analysis of Hela cells using KCND2 mouse mAb (green) and negative control (red).

Western Blot: Kv4.2 Antibody (5B11B9) [NBP2-52497] - Analysis using KCND2 mAb against HEK293 (1) and KCND2 (AA: 27-184)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Kv4.2 Antibody (5B11B9) [NBP2-52497] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 5B11B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Kv4.2 Antibody (5B11B9) - BSA Free Summary

• Description: Novus Biologicals Mouse Kv4.2 Antibody (5B11B9) - BSA Free (NBP2-52497) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Kv4.2 (AA: 27-184) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCND2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 70.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Kv4.2 Antibody (5B11B9) - BSA Free

• potassium voltage-gated channel, Shal-related subfamily, member 2
• RK5
• voltage-gated potassium channel Kv4.2
• voltage-sensitive potassium channel

Background

Kv4.2, a voltage-gated K+ (Kv) channel subunit, has been suggested to be the key component of the subthreshold A-type K+ currents (I(SA)s) recorded from the specific subcellular compartments of certain CNS neurons (1). A-type channels, encoded by the pore-forming alpha-subunits of the Kv4.x family, are particularly important in regulating membrane excitability in the CNS and the heart. Kv4.2 is a Shal-type potassium channel that passes an A-type current and is localized to dendrites and cell bodies in the hippocampus (2). Deletion of the Kv4.2 gene and a loss of Kv4.2 protein resulted in a specific and near-complete elimination of A-type K+ currents from the apical dendrites of CA1 pyramidal neurons. The absence of dendritic Kv4.2-encoded A-type K+ currents led to an increase of bAP amplitude and an increase of concurrent Ca2+ influx (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCND2