KRT9 Overexpression Lysate (Native) Summary
| Immunogen |
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_000217. The protein contains a C-terminal DDK tag. |
| Specificity |
Homo sapiens KRT9, mRNA. |
| Gene |
KRT9 |
Applications/Dilutions
| Application Notes |
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug). |
| Theoretical MW |
61.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
RIPA buffer |
Lysate Details for KRT9
| Type |
Overexpression |
| Protein State |
Native |
Notes
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for KRT9 Overexpression Lysate (Native)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Blogs on KRT9
![Western Blot: KRT9 Overexpression Lysate (Adult Normal) [NBP2-09046] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KRT9.](http://images.novusbio.com/fullsize/KRT9 Overexpression Lysate (Adult Normal)-Western Blot-NBP2-09046-img0001.jpg "Western Blot: KRT9 Overexpression Lysate (Adult Normal) [NBP2-09046] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KRT9.")
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Western-Blot-NBP1-30141-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).](http://images.novusbio.com/fullsize2/pan-Cytokeratin-Antibody-(AE1-+-AE3)-Immunohistochemistry-Paraffin-NBP2-29429-img0008.jpg)
![Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Pan Cytokeratin was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 1.7 ug/mL of mouse anti-pan Cytokeratin monoclonal (NBP2-29429, Novus Biologicals) for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize2/pan-Cytokeratin-Antibody-(AE1-+-AE3)-Immunohistochemistry-Paraffin-NBP2-29429-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - MCF-7 cells stained with Biotin conjugated version of pan Cytokeratin antibody and SAv-A488. A) SAv-A488 only at a dilution of 1:500, B) pan CK at a dilution of 1:200, C) pan CK at a dilution of 1:400, D) pan CK at a dilution of 1:800. Image from verified customer review.](http://images.novusbio.com/fullsize2/pan-Cytokeratin-Antibody-(AE1-+-AE3)-Immunocytochemistry-Immunofluorescence-NBP2-29429-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002).](http://images.novusbio.com/fullsize2/Cytokeratin-14-Antibody-(LL002)-Immunohistochemistry-Paraffin-NBP2-34270-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human prostate (10X) stained with Cytokeratin 14 MAb (LL002).](http://images.novusbio.com/fullsize2/Cytokeratin-14-Antibody-(LL002)-Immunohistochemistry-Paraffin-NBP2-34270-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human prostate (20X) stained with Cytokeratin 14 MAb (LL002).](http://images.novusbio.com/fullsize2/Cytokeratin-14-Antibody-(LL002)-Immunohistochemistry-Paraffin-NBP2-34270-img0003.jpg)
![Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.](http://images.novusbio.com/fullsize/HIST1H4F Overexpression Lysate (Adult Normal)-Western Blot-NBP2-07996-img0001.jpg)
![Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded esophageal cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/H4-n-Antibody-(3E7D9)-Immunohistochemistry-NBP2-52487-img0003.jpg)
![Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded colon cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/H4-n Antibody (3E7D9)-Immunohistochemistry-NBP2-52487-img0004.jpg)
![Immunofluorescence: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize2/H4-n-Antibody-(3E7D9)-Immunofluorescence-NBP2-52487-img0001.jpg)
![Western Blot: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - KRT4 monoclonal antibody (M01), clone 5H5 Analysis of KRT4 expression in A-431.](http://images.novusbio.com/fullsize2/Cytokeratin 4 Antibody (5H5)-Western Blot-H00003851-M01-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - Analysis of monoclonal antibody to KRT4 on A-431 cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/Cytokeratin-4-Antibody-(5H5)-Immunocytochemistry-Immunofluorescence-H00003851-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 4 Antibody (5H5) [H00003851-M01] - Analysis of monoclonal antibody to KRT4 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/Cytokeratin-4-Antibody-(5H5)-Immunohistochemistry-Paraffin-H00003851-M01-img0008.jpg)
![Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).](http://images.novusbio.com/fullsize2/HP1-alpha-Antibody-Western-Blot-NB110-40623-img0002.jpg)
![Immunohistochemistry: HP1 alpha Antibody [NB110-40623] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize2/HP1 alpha Antibody-Immunohistochemistry-NB110-40623-img0015.jpg)
![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - FFPE section of human ovarian tumor. Affinity purified rabbit anti-BIRC6/Apollon dilution of 1:250.](http://images.novusbio.com/fullsize2/HP1-alpha-Antibody-Immunohistochemistry-Paraffin-NB110-40623-img0001.jpg)
![Flow Cytometry: Cytokeratin 10 Antibody (SPM261) [NBP2-32962] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-32962. An intracellular stain was performed on HeLa cells with Cytokeratin 10 antibody (SPM261) NBP2-34752 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 microgram of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/Cytokeratin-10-Antibody-(SPM261)-Flow-Cytometry-NBP2-32962-img0001.jpg)
![Western Blot: CCDC6 Antibody [NBP1-85351] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/CCDC6-Antibody-Western-Blot-NBP1-85351-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CCDC6 Antibody [NBP1-85351] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize2/CCDC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85351-img0013.jpg)
![Immunohistochemistry-Paraffin: CCDC6 Antibody [NBP1-85351] - Staining in human fallopian tube and skeletal muscle tissues using anti-CCDC6 antibody. Corresponding CCDC6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/CCDC6-Antibody-Immunohistochemistry-Paraffin-NBP1-85351-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of 1 month old zebrafish embryo](http://images.novusbio.com/fullsize2/Cytokeratin 18 Antibody (RGE53)-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0006.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Frozen section of swine liver hepatocytes](http://images.novusbio.com/fullsize2/Cytokeratin 18 Antibody (RGE53)-Immunohistochemistry-Frozen-NBP1-97715-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (RGE53) [NBP1-97715] - Staining of a 2 days old zebrafish embryo. Left panel: DAPI-staining of cell nuclei, providing an overview of the tissue section used for immunostaining](http://images.novusbio.com/fullsize2/Cytokeratin 18 Antibody (RGE53)-Immunocytochemistry-Immunofluorescence-NBP1-97715-img0004.jpg)
![Western Blot: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 16.](http://images.novusbio.com/fullsize2/Cytokeratin 16 Antibody (2H8)-Western Blot-NBP2-45538-img0018.jpg)
![Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize2/Cytokeratin-16-Antibody-(2H8)-Immunohistochemistry-NBP2-45538-img0017.jpg)
![Immunohistochemistry: Cytokeratin 16 Antibody (2H8) [NBP2-45538] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize2/Cytokeratin-16-Antibody-(2H8)-Immunohistochemistry-NBP2-45538-img0001.jpg)
![Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Cytokeratin-8-Antibody-Western-Blot-NBP2-16094-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in iced-cold MeOH for 5 min. Green: KRT8 protein stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize2/Cytokeratin 8 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16094-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/Cytokeratin 8 Antibody-Immunohistochemistry-Paraffin-NBP2-16094-img0003.jpg)
![Western Blot: HOXB2 Antibody [NBP2-56905] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize2/HOXB2 Antibody-Western Blot-NBP2-56905-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HOXB2 Antibody [NBP2-56905] - Staining of human cell line HEK 293 shows localization to nucleus.](http://images.novusbio.com/fullsize2/HOXB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56905-img0001.jpg)
![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Western blot analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Western-Blot-NBP2-22194-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunohistochemistry-Paraffin-NBP2-22194-img0005.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Western-Blot-NBP1-30141-img0012.jpg "Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry: KRT9 Antibody [NBP1-94099] - Staining of human skin shows strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/images2/KRT9-Antibody-Immunohistochemistry-NBP1-94099-img0003.jpg "Immunohistochemistry: KRT9 Antibody [NBP1-94099] - Staining of human skin shows strong cytoplasmic positivity in epidermal cells.")
![Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).](http://images.novusbio.com/images2/pan-Cytokeratin-Antibody-(AE1-+-AE3)-Immunohistochemistry-Paraffin-NBP2-29429-img0008.jpg "Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).")