KPNA6 Overexpression Lysate (Native)

Images

 
Western Blot: KPNA6 Overexpression Lysate (Adult Normal) [NBL1-12374] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KPNA6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KPNA6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203926 and based on accession number NM_012316. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens karyopherin alpha 6 (importin alpha 7) (KPNA6), mRNA.
Gene
KPNA6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for KPNA6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KPNA6 Overexpression Lysate (Native)

  • importin alpha 7 subunit
  • importin subunit alpha-7
  • importin-alpha-S2
  • IPOA7FLJ11249
  • karyopherin alpha 6 (importin alpha 7)
  • Karyopherin subunit alpha-6
  • KPNA7
  • MGC17918

Background

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. The protein encoded by this gene is a member of the importin alpha family. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for KPNA6 Lysate (NBL1-12374) (0)

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Video Protocols

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Bioinformatics

Gene Symbol KPNA6