KLC1 Overexpression Lysate

Images

 
Western Blot: KLC1 Overexpression Lysate (Adult Normal) [NBL1-12308] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KLC1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KLC1 Overexpression Lysate Summary

Description
Transient overexpression lysate of kinesin light chain 1 (KLC1), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC203467 and based on accession number NM_005552. The protein contains a C-MYC/DDK Tag.
Gene
KLC1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
63.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KLC1 Overexpression Lysate

  • hKLC1B
  • hKLC1G
  • hKLC1J
  • hKLC1S
  • kinesin 2 60/70kDa
  • kinesin 2
  • kinesin light chain 1
  • KLC 1
  • KLChKLC1N
  • KNS2AhKLC1P
  • KNS2hKLC1R
  • medulloblastoma antigen MU-MB-2.50
  • MGC15245

Background

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named 'kinesin 2', this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined. [provided by RefSeq]. Transcript Variant: This variant (1) has an alternate 3' sequence, as compared to variant 3. The resulting isoform (1, also known as C) has a shorter and distinct C-terminus, as compared to isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for KLC1 Lysate (NBL1-12308) (0)

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Bioinformatics

Gene Symbol KLC1