Kir3.3 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Kir3.3 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human Kir3.3.

  Source: E. coli

  Amino Acid Sequence: RLDAHLYWSIPSRLDEKVEEE

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: KCNJ9
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-57515.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Kir3.3 Recombinant Protein Antigen

• G protein-activated inward rectifier potassium channel 3
• G protein-coupled inward rectifier potassium channel
• GIRK-3
• GIRK3KIR3.3
• Inward rectifier K(+) channel Kir3.3
• inwardly rectifier K+ channel KIR3.3
• Kir3.3
• Potassium channel, inwardly rectifying subfamily J member 9
• potassium inwardly-rectifying channel, subfamily J, member 9

Background

The Kir3.3 (KCNJ9) encodes for a 393 amino acid long, 44 kDA, G-protein activated inward rectifier potassium channel protein that creates heteromultimeric pore-forming complexes. The Kir3.3 gene has been investigated in generalized epilepsy, meniere's disease, pertussis, lung cancer, schizophrenia, and diabetes mellitus. It interacts with the DRD4, KCNJ6, ADRB2, KCNJ3, and DRD2 genes in potassium transporting pathways (inward and outward), G-Beta gamma signaling, neurotransmitter receptor binding and downward transmission in the postsynaptic cell, and ion currents in synaptic transmission.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: KCNJ9