Kif4A Overexpression Lysate (Denatured)

Images

 
Lane 1: KIF4A transfected lysate (124.08 KDa) Lane 2: Non-transfected lysate.
KIF4A transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA

Order Details

Kif4A Overexpression Lysate (Denatured) Summary

Description
Pasmid: pCMV-KIF4A full-length
Gene
KIF4A

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).

Applications/Dilutions

Application Notes
ELISA usage reported by customer review.
Reviewed Applications
Read 1 Review rated 4
using
H00024137-T01 in the following application:

Lysate Details for Kif4A

Type
Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-KIF4A antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Kif4A Overexpression Lysate (Denatured)

  • chromokinesin-A
  • chromosome-associated kinesin KIF4A
  • FLJ12530
  • FLJ12655
  • FLJ14204
  • FLJ20631
  • HSA271784
  • KIF4G1
  • KIF4KIF4-G1
  • kinesin family member 4A

Background

Kinesins, such as KIF4A, are microtubule-based motor proteins that generate directional movement along microtubules. They are involved in many crucial cellular processes, including cell division (Zhu and Jiang, 2005 [PubMed 15625105]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Kif4A Lysate (H00024137-T01) (0)

There are no publications for Kif4A Lysate (H00024137-T01).
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Reviews for Kif4A Lysate (H00024137-T01) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

Reviews using H00024137-T01:
Filter by Applications
ELISA
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
ELISA Kif4A H00024137-T01
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reviewed by:
Anonymous
ELISA Human 07/07/2010
View

Summary

ApplicationELISA
Sample TestedHuman antibody
SpeciesHuman
Lot10123022
CommentsLysate used to coat plates was optimized empirically against various concentrations of human antibody detected with goat anti-human IgG-HRP.

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: PBS-Tween, Dilution Ratio: 1/10, Incubation Time: 1 hour, Incubation Temp: room temperature

Secondary Antibody

Secondary DescriptionSecondary Ab: Biotinylated Anti-Human IgG

Details

Detection NotesWavelength: 450-650nM
ELISA Plate DetailsELISA Detection Method: SA-HRP, TMB substrate, ELISA Sample Concentration: 1000 to 0.001 ng/mL

Comments

CommentsLysate used to coat plates was optimized empirically against various concentrations of human antibody detected with goat anti-human IgG-HRP.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Kif4A Lysate (H00024137-T01) (0)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
07/07/2010
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol KIF4A

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