Kif4A Antibody

Immunocytochemistry/Immunofluorescence: Kif4A Antibody [NB100-1053] - Staining (green) of 4% formaldahyde-fixed dividing HeLa with endogenously expressed Histone H2B-GFP fusion as DNA marker (red). Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, WIMM, JR Hospital Oxford.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kif4A Antibody Summary

• Description: Novus Biologicals Goat Kif4A Antibody (NB100-1053) is a polyclonal antibody validated for use in ELISA and ICC/IF. Anti-Kif4A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SFFSGCSPIEEEAH corresponding to C-Terminus according to NP_036442.3.
• Localization: Nuclear
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KIF4A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:2000
  • Peptide ELISA Detection limit 1:64000
• Application Notes: Kif4A antibody validated for IF (on HeLa cells, methanol-fixed and BSA-blocked) and WB (HeLa cells) from verified customer reviews.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kif4A Antibody

• chromokinesin-A
• chromosome-associated kinesin KIF4A
• FLJ12530
• FLJ12655
• FLJ14204
• FLJ20631
• HSA271784
• KIF4G1
• KIF4KIF4-G1
• kinesin family member 4A

Background

KIF4A is required for mitotic chromosomal positioning and bipolar spindle stabilization. It is located in the nucleus and is associated with mitotic chromosomes. It is highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kif4A Antibody (NB100-1053)(1)

Publication using NB100-1053

• Sekine Y, Okada Y, Noda Y et al. A novel microtubule-based motor protein (KIF4) for organelle transports, whose expression is regulated developmentally. J Cell Biol 1994-10-01 [PMID: 7929562]

Bioinformatics

• Gene Symbol: KIF4A
• Entrez:
  • 24137(Human)
• Uniprot:
  • O95239(Human)