KDM2B Antibody (6F6G11) - BSA Free

Western Blot: KDM2B Antibody (6F6G11) [NBP2-52467] - Analysis using FBXL10 mAb against human FBXL10 (AA: 457-555) recombinant protein. (Expected MW is 36.8 kDa)

Flow Cytometry: KDM2B Antibody (6F6G11) [NBP2-52467] - Analysis of HeLa cells using FBXL10 mouse mAb (green) and negative control (red).

Western Blot: KDM2B Antibody (6F6G11) [NBP2-52467] - Analysis using FBXL10 mouse mAb against A549 (1) cell lysate.

Western Blot: KDM2B Antibody (6F6G11) [NBP2-52467] - Analysis using FBXL10 mAb against HEK293 (1) and FBXL10 (AA: 457-555)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: KDM2B Antibody (6F6G11) [NBP2-52467] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 6F6G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

KDM2B Antibody (6F6G11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human KDM2B (AA: 457-555) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDM2B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 152.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KDM2B Antibody (6F6G11) - BSA Free

• [Histone-H3]-lysine-36 demethylase 1B
• CXXC2
• CXXC2F-box protein FBL10
• CXXC-type zinc finger protein 2
• FBL10
• F-box and leucine-rich repeat protein 10JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein
• F-box protein FBL10
• FBXL10
• JHDM1B
• JHDM1BF-box/LRR-repeat protein 10
• JmjC domain-containing histone demethylation protein 1B
• jumonji C domain-containing histone demethylase 1B
• Jumonji domain-containing EMSY-interactor methyltransferase motif protein
• KDM2B
• lysine (K)-specific demethylase 2B
• lysine-specific demethylase 2B
• NDY1
• PCCX2
• PCCX2EC 1.14.11.27
• protein containing CXXC domain 2
• Protein JEMMA
• Protein-containing CXXC domain 2

Background

KDM2B encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM2B