KDEL Antibody (2C1) - BSA Free

Western Blot: KDEL Antibody (2C1) [NBP2-61926] - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Lane 1: Molecular Weight Ladder (MW). Lane 2: Anti-KDEL (NBP2-61926). Lane 3: Anti-KDEL control (SPC-109). Lane 4: Anti-PDI control (SPC-114). Lane 5: Anti-Calreticulin control (SPC-122). Lane 6: Anti-GRP78 control (SPC-107). Lane 7: Anti-GRP94 control (SPC-101).. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG:HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.

Immunohistochemistry: KDEL Antibody (2C1) [NBP2-61926] - Immunohistochemistry analysis using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1 (NBP2-61926). Tissue: kidney. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-KDEL Monoclonal Antibody (NBP2-61926) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) KDEL Antibody (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 2C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

KDEL Antibody (2C1) - BSA Free Summary

• Description: Novus Biologicals Mouse KDEL Antibody (2C1) - BSA Free (NBP2-61926) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
• Specificity: Detects KDEL tagged proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POGLUT2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:25
  • Western Blot 1:1000
• Application Notes: A 1:1000 dilution of this antibody was sufficient for detection of KDEL in 10 ug of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KDEL Antibody (2C1) - BSA Free

• Endoplasmic reticulum resident protein 58
• EP58KDEL1
• ER protein 58
• ERp58
• KDEL (Lys-Asp-Glu-Leu) containing 1
• KDEL motif-containing 1
• KDEL motif-containing protein 1
• MGC5302

Background

he endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POGLUT2