KDEL Antibody (2C1)

Images

 
Western Blot: KDEL Antibody (2C1) [NBP2-61926] - analysis of Human HeLa cell lysates showing detection of Multiple KDEL protein using Mouse Anti-KDEL Monoclonal Antibody, Clone 2C1. Lane 1: Molecular Weight Ladder (MW). ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
2C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

KDEL Antibody (2C1) Summary

Immunogen
KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Specificity
Detects KDEL tagged proteins.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
POGLUT2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:25
Application Notes
A 1:1000 dilution of this antibody was sufficient for detection of KDEL in 10 ug of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for KDEL Antibody (2C1)

  • Endoplasmic reticulum resident protein 58
  • EP58KDEL1
  • ER protein 58
  • ERp58
  • KDEL (Lys-Asp-Glu-Leu) containing 1
  • KDEL motif-containing 1
  • KDEL motif-containing protein 1
  • MGC5302

Background

he endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-61926AF405
Alexa Fluor 488 NBP2-61926AF488
Alexa Fluor 647 NBP2-61926AF647
Alexa Fluor 700 NBP2-61926AF700
Biotin NBP2-61926B
DyLight 350 NBP2-61926UV
DyLight 405 NBP2-61926V
DyLight 488 NBP2-61926G
DyLight 550 NBP2-61926R
DyLight 650 NBP2-61926C
DyLight 680 NBP2-61926FR
DyLight 755 NBP2-61926IR
HRP NBP2-61926H

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Array NBP2-61926

Bioinformatics Tool for KDEL Antibody (NBP2-61926)

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Diseases for KDEL Antibody (NBP2-61926)

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol POGLUT2