Recombinant Human KCNMB3 His Protein

SDS-Page: KCNMB3 Recombinant Protein [NBP2-51813] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human KCNMB3 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 82-207 of Human KCNMB3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSKPFMLSI QREESTCTAI HTDIMDDWLD CAFTCGVHCH GQGKYPCLQV FVNLSHPGQK ALLHYNEEAV QINPKCFYTP KCHQDRNDLL NSALDIKEFF DHKNGTPFSC FYSPASQSED VILIKKYDQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: KCNMB3
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 16.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human KCNMB3 His Protein

• BK channel subunit beta-3
• BKbeta3
• Hbeta3
• KCNMB2
• KCNMBL
• potassium large conductance calcium-activated channel, subfamily M beta member3
• slo-beta-3
• subfamily M subunit beta-3
• voltage and Ca2+ activated potassium channel Maxi K beta 3subunit

Background

MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. The protein encoded by this gene is an auxiliary beta subunit which may partially inactivate or slightly decrease the activation time of MaxiK alpha subunit currents. At least four transcript variants encoding four different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: KCNMB3