KCNMB3 Antibody (S40B-18)

Western Blot: KCNMB3 Antibody (S40B-18) [NBP2-12916] - analysis of Rat brain membrane lysate showing detection of KCNMB3 Potassium Channel protein using Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody, Clone S40B-18 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: KCNMB3 Antibody (S40B-18) [NBP2-12916] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Accumulation at the edges of the cell, ruffling edges.

Immunohistochemistry-Paraffin: KCNMB3 Antibody (S40B-18) [NBP2-12916] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-KCNMB3 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Beautiful filaggrinlike staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, MA
• Clone: S40B-18
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

KCNMB3 Antibody (S40B-18) Summary

• Immunogen: Fusion protein amino acids 1-49 (unique N-terminus) of mouse BKBeta3a
• Localization: Membrane
• Specificity: Detects approx 32 kDa. No cross-reactivity against BKBeta1, BKBeta2, BKBeta3b or BKBeta4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNMB3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1-10 ug/ml
• Application Notes: 1 ug/ml of BK Beta 3a Antibody was sufficient for detection of BK Beta3a in 10 ug of COS cell (lysate) transiently transfected with BKbeta3a by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KCNMB3 Antibody (S40B-18)

• BK channel subunit beta-3
• BKbeta3
• Hbeta3
• KCNMB2
• KCNMBL
• potassium large conductance calcium-activated channel, subfamily M beta member3
• slo-beta-3
• subfamily M subunit beta-3
• voltage and Ca2+ activated potassium channel Maxi K beta 3subunit

Background

1 ug/mL was sufficient for detection of BK Beta3a in 10ug of COS cell (lysate) transiently transfected with BKbeta3a by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCNMB3 Antibody (NBP2-12916)(2)

Publications using NBP2-12916

• Imari K, Harada Y, Zhang J et al. KCNMB3 in spinal microglia contributes to the generation and maintenance of neuropathic pain in mice Int. J. Mol. Med. 2019-07-19 [PMID: 31364720]
• Hayashi Y, Morinaga S, Zhang J et al. BK channels in microglia are required for morphine-induced hyperalgesia. Nat Commun 2016-05-31 [PMID: 27241733] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: KCNMB3