KCNK10 Overexpression Lysate

Images

 
Western Blot: KCNK10 Overexpression Lysate (Adult Normal) [NBL1-12177] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KCNK10.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KCNK10 Overexpression Lysate Summary

Description

KCNK10 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC223229

Accession#: NM_138317

Protein Tag: C-MYC/DDK
Gene
KCNK10

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
60.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

The lysate was created in HEK293T cells, using plasmid ID RC223229 and based on accession number NP_612190. The protein contains a C-terminal DDK tag. HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KCNK10 Overexpression Lysate

  • 2P domain potassium channel TREK2
  • K2p10.1
  • KCNK10
  • Outward rectifying potassium channel protein TREK-2
  • potassium channel subfamily K member 10
  • potassium channel TREK-2
  • potassium channel, subfamily K, member 10
  • TREK-2 K(+) channel subunit
  • TREK2
  • TREK-2
  • TREK2FLJ43399
  • TWIK-related K+ channel 2

Background

The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (2, also known as TREK2 splice variant c) has a different 5' terminal exon compared to transcript variant 1, and encodes a slightly longer isoform (2) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol KCNK10