KCNJ1 Antibody

Western Blot: KCNJ1 Antibody [NBP1-36965] - (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

KCNJ1 Antibody Summary

• Description: Novus Biologicals Goat KCNJ1 Antibody (NBP1-36965) is a polyclonal antibody validated for use in WB and ELISA. Anti-KCNJ1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DQININFVVDAGNEN corresponding to internal region according to NP_722448.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize reported isoforms NP_722449.2 and NP_000211. The following reported variants represent identical protein: NP_722451.1, NP_722449.2, NP_722450.1, NP_722448.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KCNJ1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Theoretical MW: 44.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KCNJ1 Antibody

• ATP-regulated potassium channel ROM-K
• ATP-sensitive inward rectifier potassium channel 1
• inwardly rectifying K+ channel
• KIR1.1
• potassium inwardly-rectifying channel, subfamily J, member 1
• ROMK1inwardly rectifying subfamily J member 1

Background

FUNCTION: In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.; Tissue specificity: In the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver.; Subcellular location: Membrane, Multi-pass membrane protein.; Involvement in disease: Defects in KCNJ1 are the cause of Bartter syndrome type 2 (BS2) also termed hyperprostanglandin E syndrome 2. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS2 is a life-threatening condition beginning in utero, with marked fetal polyuria that leads to polyhydramnios and premature delivery. Another hallmark of BS2 is a marked hypercalciuria and, as a secondary consequence, the development of nephrocalcinosis and osteopenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCNJ1 Antibody (NBP1-36965)(1)

Publication using NBP1-36965

• Ji W, Foo JN, O'Roak BJ et al. Rare independent mutations in renal salt handling genes contribute to blood pressure variation. Nat Genet 2008-05-01 [PMID: 18391953]

Bioinformatics

• Gene Symbol: KCNJ1
• Entrez:
  • 3758(Human)
  • 56379(Mouse)
  • 24521(Rat)
• Uniprot:
  • NP_000211.1(Human)