KChIP2 Overexpression Lysate

Images

 
Western Blot: KChIP2 Overexpression Lysate (Adult Normal) [NBL1-12159] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KChIP2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

KChIP2 Overexpression Lysate Summary

Description
Transient overexpression lysate of Kv channel interacting protein 2 (KCNIP2), transcript variant 3 The lysate was created in HEK293T cells, using Plasmid ID RC203823 and based on accession number NM_173192. The protein contains a C-MYC/DDK Tag.
Gene
KCNIP2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KChIP2 Overexpression Lysate

  • A-type potassium channel modulatory protein 2
  • cardiac voltage gated potassium channel modulatory subunit
  • Cardiac voltage-gated potassium channel modulatory subunit
  • DKFZp566L1246
  • KChIP2
  • KCHIP2Kv channel-interacting protein 2
  • Kv channel interacting protein 2
  • MGC17241
  • potassium channel interacting protein 2
  • Potassium channel-interacting protein 2

Background

This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belongs to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified from this gene. [provided by RefSeq]. Transcript Variant: This variant (3), also known as KChIP2.1, lacks an in-frame segment in the coding region, as compared to variant 1. Isoform 3 is thus shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol KCNIP2