Goat anti-Human Kappa Light Chain Secondary Antibody

Western Blot: Goat anti-Human Kappa Light Chain Secondary Antibody [Unconjugated] (Pre-adsorbed) [NBP2-60691] - Lane 1: Human ?. Lane 2: Human lambda. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Human ?. Other band(s): None. Image using the Biotin form of this antibody.

Product Discontinued

Goat anti-Human Kappa Light Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Human kappa light chain
• Specificity: Specificity for kappa chain was confirmed by ELISA minimal cross reactivity against other human heavy or lambda light chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGKC
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:20000
  • Immunohistochemistry 1:1000-1:5000
  • Western Blot 1:2000-1:10000
• Application Notes: This product has been tested by ELISA and western blot and is suitable for immunohistochemistry and immunoassays where specificity to the immunoglobulin kappa subunit is desired. Optimal concentrations in immunoassays should be determined by the researcher.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Goat anti-Human Kappa Light Chain Secondary Antibody

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGKC