Kappa Light Chain Antibody (RM103)

Western Blot: Kappa Light Chain Antibody (RM103) [Unconjugated] [NBP2-62003] - Western blot of nonreduced(-) and reduced(+) mouse IgG1 kappa and IgG1 lambda, using NBP2-62003. This antibody reacts to nonreduced IgG1k (~150 kDa), and slightly reacts to reduced kappa light chain (~25 kDa).

ELISA: Kappa Light Chain Antibody (RM103) - Azide and BSA Free [NBP2-62003] - A titer ELISA of mouse IgG1k. The plate was coated with different amounts of mouse IgG1k. A serial dilution of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA: Kappa Light Chain Antibody (RM103) - Azide and BSA Free [NBP2-62003] - ELISA of mouse immunoglobulins shows RM103 reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG (H + L), rat IgG (H + L), or goat IgG (H + L). The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM103 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

• Reactivity: Mu
• Applications: WB, ELISA
• Clone: RM103
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Kappa Light Chain Antibody (RM103) Summary

• Description: Novus Biologicals Rabbit Kappa Light Chain Antibody (RM103) (NBP2-62003) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse IgG
• Specificity: This antibody reacts to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lambda light chain, human IgG, rat IgG, or goat IgG.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGKC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.005ug/ml-0.2ug/ml
  • Western Blot 0.1ug/ml-0.5ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Kappa Light Chain Antibody (RM103)

• HCAK1
• IGKCD
• immunoglobulin kappa constant
• Km
• MGC111575
• MGC62011
• MGC72072
• MGC88770
• MGC88771
• MGC88809

Background

Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non-Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin-like domain. The EU sequence has the INV allotypic marker, Ala-45 and Val-83. The ROY sequence has the INV allotypic marker, Ala-45 and Leu-83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGKC