Kappa Light Chain Antibody (H16-E)

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Immunohistochemistry-Paraffin: Kappa Light Chain Antibody (H16-E) [NBP1-30140] - Human tissue (4 um section) stained with anti - Human Kappa light chain (DB 038) monospecific clonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
H16-E
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Kappa Light Chain Antibody (H16-E) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide derived from C-terminal sequence of human kappa light chain IgG. Antibody recognizes the epitope between Gly92 - Gly104.
Specificity
This antibody is specific to the C terminus of Human IGKC
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IGKC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
Paraffin embedded sections. For 0.5ml and 1.0ml sizes, use recommended dilutions. For 7.0ml and 15.0ml sizes, products should be used undiluted.
Publications
Read Publication using NBP1-30140.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris/HCl (pH 8.0) and 20 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

The 7mL and 15 mL sizes that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Alternate Names for Kappa Light Chain Antibody (H16-E)

  • HCAK1
  • IGKCD
  • immunoglobulin kappa constant
  • Km
  • MGC111575
  • MGC62011
  • MGC72072
  • MGC88770
  • MGC88771
  • MGC88809

Background

Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGKC
Entrez
Uniprot