Kallikrein 13 Antibody - BSA Free

Western Blot: Kallikrein 13 Antibody [NB200-139] - Analysis of KLK-L4 in LNCaP cells using this antibody at 1:1000 dilution

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Kallikrein 13 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Kallikrein 13 Antibody - BSA Free (NB200-139) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Kallikrein 13 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Residues 262-277 [IRKYETQQQKWLKGPQ] of the human KLK-L4 protein.
• Specificity: Reacts with residues 262-277 [IRKYETQQQKWLKGPQ] of the human KLK-L4 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLK13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500- 1:1000
• Application Notes: For Western Blotting, use at a dilution of 1:500 - 1:1000. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry-frozen and paraffin.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human.
Others not specified

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kallikrein 13 Antibody - BSA Free

• DKFZp586J1923
• EC 3.4.21
• EC 3.4.21.-
• Kallikrein 13
• Kallikrein-like protein 4
• kallikrein-related peptidase 13
• KLK13
• KLK-L4kallikrein-13
• KLKL4kallikrein-like gene 4

Background

Kallikreins are a subgroup of serine proteases and are implicated in carcinogenesis. The novel KLK gene, KLK-L4, is expressed in a variety of tissues including prostate, salivary gland, breast, and testis. Its expression is regulated by steroid hormones in breast cancer cell lines may be involved in the pathogenesis and/or progression of breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 13 Antibody (NB200-139)(1)

Publication using NB200-139

• Gruba N, Rachubik P, Piwkowska A, Lesner A Analysis of urinary kallikrein-related peptidase 13 for monitoring bladder cancer Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 2021-10-27 [PMID: 34704886]

FAQs for Kallikrein 13 Antibody (NB200-139). (Showing 1 - 1 of 1 FAQ).

1. Could you please provide us a WB image using #NB200-139 on human samples? Any special protocol for its use in WB?
   • The Western blot image in human cells for NB200-139 can be found on the product datasheet. The dilution range for this product in Western blot that we recommend starting with is 1:500-1:1000. The user needs to optimize the conditions in their own experimental setting however for a general guideline, please see our support section for our general Western Blot Protocol.

Bioinformatics

• Gene Symbol: KLK13
• Entrez:
  • 26085(Human)
• Uniprot:
  • Q9UKR3()