Kallikrein 12 Antibody [Unconjugated]

Kallikrein 12 was detected in immersion fixed paraffin-embedded sections of Human Duodenum using Sheep Anti-Human Kallikrein 12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3095) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells in intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Kallikrein 12 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Kallikrein 12
  Ala18-Asn248
  Accession # Q9UKR0
• Specificity: Detects human Kallikrein 12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Kallikrein 8, rhKallikrein 10, rhKallikrein 11, and rhKallikrein 15 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KLK12
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Kallikrein 12 Antibody [Unconjugated]

• DKFZp686H1078
• EC 3.4.21
• EC 3.4.21.-
• Kallikrein 12
• Kallikrein-like protein 5
• kallikrein-related peptidase 12
• KLK12
• KLK-L5
• KLKL5kallikrein-12
• KLK-L5MGC42603

Background

Human tissue Kallikrein 12, encoded by the KLK12 gene, is a secreted serine protease that belongs to the human tissue kallikrein family. It is present in many tissues, such as salivary gland, stomach and breast. KLK12 displays trypsin-like enzymatic activity. This activity can be inhibited by Serpin F2 (R&D Systems, Catalog # 1470-PI). The physiological functions of KLK12 still remain unclear. Its expression is modulated by steroid hormones and is down‑regulated in breast cancer (2). Human KLK12 has three splice variants resulting from alternative splicing of the 3’ end. The KLK12 produced by the R&D Systems corresponds to the full-length classical form, also known as isoform 2 (1). The amino acid sequence of human KLK12 is 80%, 77%, 71% and 66% to that of bovine, canine, mouse, and rat. The recombinant human KLK12 can be autoactivated under the conditions described in the Activity Assay Protocol. The active enzyme has the N-terminal sequence of I₂₂FNGTECGRNS.

1. Yousef, G.M. et al. (2000) Genomics. 69:331.
2. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 12 Antibody (AF3095)(4)

Publications using AF3095

• Qianyuan Li, Xiukou Zhou, Zhengyu Fang, Huamiao Zhou Knockdown of KLK12 inhibits viability and induces�apoptosis in human colorectal cancer HT-29 cell line International Journal of Molecular Medicine 8/30/2019 [PMID: 31485623]
• Qianyuan Li, Xiukou Zhou, Zhengyu Fang, Huamiao Zhou Knockdown of KLK12 inhibits viability and induces�apoptosis in human colorectal cancer HT-29 cell line International Journal of Molecular Medicine 2019-08-30 [PMID: 31485623] (Immunohistochemistry, Human)
• T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix Sci Rep, 2018-04-20;8(1):6331. 2018-04-20 [PMID: 29679011] (ICC, Human)
• En-Hao Zhao, Zhi-Yong Shen, Hua Liu, Xin Jin, Hui Cao Clinical significance of human kallikrein 12 gene expression in gastric cancer World Journal of Gastroenterology 12/7/2012 [PMID: 23236234]

Bioinformatics

• Gene Symbol: KLK12
• Uniprot:
  • Q9UKR0()