Kaiso Antibody (2B2)

Western Blot: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of ZBTB33 expression in transfected 293T cell line by ZBTB33 monoclonal antibody (M01), clone 2B2.Lane 1: ZBTB33 transfected lysate(74.6 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of monoclonal antibody to ZBTB33 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of ZBTB33 over-expressed 293 cell line, cotransfected with ZBTB33 Validated Chimera RNAi ( Cat # H00010009-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZBTB33 monoclonal antibody (M01), clone 2B2 (Cat # H00010009-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: Kaiso Antibody (2B2) [H00010009-M01] - Detection limit for recombinant GST tagged ZBTB33 is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

Kaiso Antibody (2B2) Summary

• Immunogen: ZBTB33 (AAH42753, 564 a.a. ~ 673 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QFMSSHIKSVHSQDPSGDSKLYRLHPCRSLQIRQYAYLSDRSSTIPAMKDDGIGYKVDTGKEPPVGTTTSTQNKPMTWEDIFIQQENDSIFKQNVTDGSTEFEFIIPESY
• Specificity: ZBTB33 - zinc finger and BTB domain containing 33 (2B2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZBTB33
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • RNA Inhibition
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Has also been used for RNAi Validation.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Kaiso Antibody (2B2)

• KAISOkaiso transcription factor
• transcriptional regulator Kaiso
• WUGSC:H_DJ525N14.1
• zinc finger and BTB domain containing 33
• Zinc finger and BTB domain-containing protein 33
• ZNF348kaiso
• ZNF-kaiso

Background

Kaiso is Caribbean slang for calypso music, which accompanied the late-night cloning of this gene. The deduced 672-amino acid human protein is 87% identical to the mouse protein, with mostly conservative changes. Kaiso contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. Immunoprecipitation analysis showed that Kaiso interacts with Ctnnd1 but not with Ctnnb1, Ctnna1 or Cdh1. Immunofluorescence analysis demonstrated a diffuse nuclear localization. Northern blot analysis of mouse tissues revealed ubiquitous expression of 5 to 6kb transcripts. Yeast 2 hybrid analysis indicated that the armadillo repeats of Ctnnd1 interact with a region flanking the zinc finger domain of Kaiso. By genomic sequence analysis, Daniel and Reynolds (1999) mapped the human KAISO gene to Xq23. They mapped the mouse Kaiso gene to the X chromosome also. Kaiso is a component of the human N-CoR complex. In vitro, the Kaiso/N-CoR complex binds specific CpG-rich sequences in a methylation-dependent manner. In vivo, Kaiso targets the N-CoR complex to the MTA2 gene promoter in a methylation-dependent manner. Kaiso is required for transcriptional repression of the methylated MTA2 locus. Furthermore, this repression also requires a functional N-CoR deacetylase complex, which brings about histone hypoacetylation and methylation of H3 lysine 9 to the MTA2 locus (Yoon HG et al. 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZBTB33
• OMIM:
  • 300329(Human)