JSRP1 Antibody (VF1c)

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Product Details

Summary
Reactivity GP, RbSpecies Glossary
Applications WB, IF
Clone
VF1c
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

JSRP1 Antibody (VF1c) Summary

Immunogen
Rabbit skeletal muscle triads.
Specificity
This detects Junctional Sarcoplasmic Reticulum in skeletal muscle. It does not cross-react with cardiac muscle.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
JSRP1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1500
  • Immunofluorescence 1:250
Application Notes
WB: Detects an approx. 90 kDa protein representing junctional SR in rabbit skeletal muscle extracts.IF: Staining of junctional sarcoplasmic reticulum results in increased intensity in fast (Type II) skeletal muscle over slow (Type I) skeletal muscle.

Reactivity Notes

Moderate cross-reactivity with Canine and Rat; weak cross-reactivity with Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for JSRP1 Antibody (VF1c)

  • FLJ32416
  • JP45
  • JP-45homolog of mouse skeletal muscle sarcoplasmic reticulum protein JP-45
  • junctional sarcoplasmic reticulum protein 1,2310032K21Rik
  • Junctional-face membrane protein of 45 kDa homolog
  • skeletal muscle sarcoplasmic reticulum protein JP-45

Background

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR's) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR's), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR's and RyR's. Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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JSRP1 NB120-2875

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Bioinformatics

Gene Symbol JSRP1