JSRP1 Antibody (VF1c)

Product Discontinued

JSRP1 Antibody (VF1c) Summary

• Immunogen: Rabbit skeletal muscle triads.
• Specificity: This detects Junctional Sarcoplasmic Reticulum in skeletal muscle. It does not cross-react with cardiac muscle.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: JSRP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence 1:250
  • Western Blot 1:1500
• Application Notes: WB: Detects an approx. 90 kDa protein representing junctional SR in rabbit skeletal muscle extracts.IF: Staining of junctional sarcoplasmic reticulum results in increased intensity in fast (Type II) skeletal muscle over slow (Type I) skeletal muscle.

Reactivity Notes

Moderate cross-reactivity with Canine and Rat; weak cross-reactivity with Mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for JSRP1 Antibody (VF1c)

• FLJ32416
• JP45
• JP-45homolog of mouse skeletal muscle sarcoplasmic reticulum protein JP-45
• junctional sarcoplasmic reticulum protein 1,2310032K21Rik
• Junctional-face membrane protein of 45 kDa homolog
• skeletal muscle sarcoplasmic reticulum protein JP-45

Background

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR's) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR's), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR's and RyR's. Another skeletal muscle specific 90 kDa protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JSRP1 Antibody (NB120-2875)(1)

Publication using NB120-2875

• Wette S, Lamb G, Murphy R Calpain-3 Is Not a Sodium Dependent Protease and Simply Requires Calcium for Activation International Journal of Molecular Sciences 2023-05-28 [PMID: 37298357] (WB, Rat, Human)

Bioinformatics

• Gene Symbol: JSRP1