JNK3 Antibody (4E12-1F7) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: JNK3 Antibody (4E12-1F7) [H00005602-M01] - Analysis of monoclonal antibody to MAPK10 on HeLa cell . Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF
• Clone: 4E12-1F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

JNK3 Antibody (4E12-1F7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAPK10 (AAH65516.1, 1 a.a. ~ 319 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSEEKTKNGVVKGQPSPSGAAVNSSESLPPSSSVNDISSMSTDQTLASDTDSSLEASAGPLGCCR
• Specificity: MAPK10 - mitogen-activated protein kinase 10
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAPK10
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Use in Proximity Ligation Assay reported in scientific literature (PMID: 25807892). Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for JNK3 Antibody (4E12-1F7) - Azide and BSA Free

• c-Jun N-terminal kinase 3
• EC 2.7.11
• EC 2.7.11.24
• JNK3 alpha protein kinase
• JNK3
• JNK3A
• JNK3FLJ12099
• MAP kinase 10
• MAP kinase p49 3F12
• MAPK 10
• MAPK10
• MGC50974
• mitogen-activated protein kinase 10
• p493F12
• p54bSAPK
• PRKM10FLJ33785
• SAPK1 beta
• stress activated protein kinase beta
• Stress-activated protein kinase JNK3

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JNK3 Antibody (H00005602-M01)(1)

Publication using H00005602-M01

• Salvucci O, Ohnuki H, Maric D et al. EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Nat Commun 2015-01-01 [PMID: 25807892] (PLA)

Bioinformatics

• Gene Symbol: MAPK10
• Entrez:
  • 5602(Human)
• OMIM:
  • 602897(Human)
• Uniprot:
  • AAH65516(Human)