JNK1 Antibody (SA43-06)

Western Blot: JNK1 Antibody (SA43-06) [NBP2-67302] - Analysis of JNK1+JNK2+JNK3 on hybrid fish (crucian-carp) brain tissue lysate using anti-JNK1+JNK2+JNK3 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: JNK1 Antibody (SA43-06) [NBP2-67302] - Staining JNK1+JNK2+JNK3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: JNK1 Antibody (SA43-06) [NBP2-67302] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-JNK1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: JNK1 Antibody (SA43-06) [NBP2-67302] - Analysis of JNK1+JNK2+JNK3 on different cell lysates using anti-JNK1+JNK2+JNK3 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC12 Lane 3: K562 Lane 5: HepG2 Lane 6: Human lung

Product Discontinued

JNK1 Antibody (SA43-06) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human JNK1 aa 1-423 / 427. (SwissProt: P45983 Human; SwissProt: P45984 Human; SwissProt: P53779 Human; SwissProt: Q61831 Mouse; SwissProt: Q91Y86 Mouse; SwissProt: Q9WTU6 Mouse; SwissProt: P49185 Rat; SwissProt: P49186 Rat; SwissProt: P49187 Rat)
• Localization: Cytoplasm, Nucleus, Membrane, Mitochondrion
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for JNK1 Antibody (SA43-06)

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• MAPK8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8
• PRKM8JNK
• protein kinase JNK1
• SAPK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

JNK1, also known as MAPK1, is a JNK protein subgroup familymenber of the mitogen-activated protein kinase (MAPK) superfamily and is part of a signal transduction cascade that is activated by cytokines and by exposure to environmental stresses. Components of the JNK (c-Jun N-terminal kinase) signaling pathway have been shown to interact with the insulin signaling pathway and play a pivotal role in lifespan regulation and stress resistance in many diverse organisms. Therefore, JNK1 plays a role in regulating both life span and stress resistance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK8