JNK1/2/3 Antibody

Product Discontinued

JNK1/2/3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human JNK1.
• Specificity: JNK1/2/3 (T178) detects endogenous levels of JNK1/2/3 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of JNK1/2/3 (T178) pAb in extracts from PC12 cells.
  Immunohistochemistry (IHC) analyzes of JNK1/2/3 (T178) pAb in paraffin-embedded human colon carcinoma tissue.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for JNK1/2/3 Antibody

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8JNK
• protein kinase JNK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1alpha1,JNK1beta1, JNK2alpha1, JNK2beta1 and JNK3alpha1, which represent the p46 isoforms, and JNK1alpha2, JNK1beta2, JNK2alpha2, JNK2beta2 and JNK3beta2, which represent the p54 isoforms. JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JNK1/2/3 Antibody (NBP1-19872)(1)

Publication using NBP1-19872

• Tian J, Guo X, Liu XM et al. Extracellular HSP60 induces inflammation through activating and up-regulating TLRs in cardiomyocytes. Cardiovasc Res. 2013-06-01 [PMID: 23447644] (WB, Rat)

Bioinformatics

• Gene Symbol: MAPK8
• Entrez:
  • 177460(C. elegans)
  • 5599(Human)
  • 5602(Human)
  • 116554(Rat)