Jagged 1/Jagged 2 Antibody (241002) [Allophycocyanin]

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human/Mouse Jagged 1/Jagged 2 APC-conjugated Monoclonal Antibody (Catalog # FAB1726A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse Jagged 1/Jagged 2 APC-conjugated Monoclonal Antibody (Catalog # FAB1726A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Mu
• Applications: Flow
• Clone: 241002
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Jagged 1/Jagged 2 Antibody (241002) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Jagged 2
  Met27-Asp307
  Accession # Q9Y219
• Specificity: Stains human Jagged 1 and Jagged 2 transfectants but not the parental cell line by flow cytometry. Detects endogenous human and mouse Jagged 1 and Jagged 2 by flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: JAG1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 1/Jagged 2 Antibody (241002) [Allophycocyanin]

• AGS;AGS1;AHD;AWS;CD339;CMT2HH;DCHE;HJ1;JAG1;JAGL1;Protein jagged-1
• Jagged 1/Jagged 2

Background

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

1. Shawber, C. et al. (1996) Dev. Biol. 180:370.
2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
4. Schickwann, T. et al. (2000) Blood 96:950.
5. DeHart, S. et al. (2005) Blood 105:3521.
6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jagged 1/Jagged 2 Antibody (FAB1726A)(2)

Publications using FAB1726A

• AJ Parry, M Hoare, D Bihary, R Hänsel-Her, S Smith, K Tomimatsu, E Mannion, A Smith, P D'Santos, IA Russell, S Balasubram, H Kimura, SA Samarajiwa, M Narita NOTCH-mediated non-cell autonomous regulation of chromatin structure during senescence Nat Commun, 2018-05-09;9(1):1840. 2018-05-09 [PMID: 29743479] (Flow Cytometry, Human)
• M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita NOTCH1 mediates a switch between two distinct secretomes during senescence Nat. Cell Biol, 2016-08-15;18(9):979-92. 2016-08-15 [PMID: 27525720] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: JAG1
• Uniprot:
  • Q9Y219(Human)