IZUMO1 Antibody [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Janelia Fluor 525

IZUMO1 Antibody [Janelia Fluor® 525] Summary

• Immunogen: IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1. The immunogen is located within amino acids 170 - 220 of IZUMO1. Amino Acid Squence: ELNWHQASEGLTDYSFY
• Specificity: IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IZUMO1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat: (82%), Mouse: (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IZUMO1 Antibody [Janelia Fluor® 525]

• FLJ61440
• izumo sperm-egg fusion 1
• izumo sperm-egg fusion protein 1
• IZUMO
• IZUMO1
• MGC34799
• OBF
• Oocyte binding/fusion factor
• Sperm-specific protein izumo

Background

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasmamembrane binding and fusion (Inoue et al., 2005 (PubMed 15759005)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IZUMO1