ITPR2 Antibody

Immunocytochemistry/Immunofluorescence: ITPR2 Antibody [NBP2-76937] - ICC staining ITPR2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: ITPR2 Antibody [NBP2-76937] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

Flow Cytometry: ITPR2 Antibody [NBP2-76937] - Flow cytometric analysis of HepG2 cells with ITPR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ITPR2 Antibody [NBP2-76937] - ICC staining ITPR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: ITPR2 Antibody [NBP2-76937] - ICC staining ITPR2 in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: ITPR2 Antibody [NBP2-76937] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: ITPR2 Antibody [NBP2-76937] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

Immunohistochemistry: ITPR2 Antibody [NBP2-76937] - Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ITPR2 antibody. Counter stained with hematoxylin.

Dot Blot: ITPR2 Antibody [NBP2-76937] - Dot blot analysis of anti-ITPR2 on PVDF. 0-4ug antigens were given in this test. Anti-ITPR2 antibody was diluted with 1/500.

Product Discontinued

ITPR2 Antibody Summary

• Immunogen: Recombinant protein within human ITPR2 aa 1100-1450. (SwissProt: Q14571 Human; SwissProt: Q9Z329 Mouse; SwissProt: P29995 Rat)
• Localization: Endoplasmic reticulum membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITPR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:2000-1:5000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 -1:200
• Theoretical MW: 308 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ITPR2 Antibody

• inositol 14,5-triphosphate receptor, type 2
• inositol 14,5-trisphosphate receptor type 2
• InsP3R2
• IP3 receptor isoform 2
• IP3 receptor
• IP3R 2
• IP3R2
• ITPR2
• Type 2 inositol 14,5-trisphosphate receptor
• Type 2 InsP3 receptor

Background

Inositol 1,4,5-triphosphate (IP3) receptors are a form of ligand-gated ion channels that are activated by cytosolic Ca2+ and IP3. They are localized to intracellular membranes, such as the endoplasmic reticulum, and mediate the mobilization of intracellular Ca2+ stores. IP3 receptors play an important role in intracellular Ca2+ signaling in a variety of cell types. There are three IP3 receptor subtypes; IP3R1, IP3R2 and IP3R3, which exist as homo- and heterotetramers. All subtypes are closely associated with calmodulin and FK506-binding protein and are modulated through phosphorylation by PKA, PKC, PKG and CaMKII.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITPR2