IRS4 Antibody (EP907Y)

Product Discontinued

IRS4 Antibody (EP907Y) Summary

• Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human IRS-4 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IRS4
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-100
  • Immunoprecipitation 1:20
  • Western Blot 1:1000-10000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 133 - 160kDa.

Reactivity Notes

Reacts with: Human Does not react with Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for IRS4 Antibody (EP907Y)

• insulin receptor substrate 4,160 kDa phosphotyrosine protein
• IRS-4pp160
• Phosphoprotein of 160 kDa
• PY160

Background

A new member of the IRS family, IRS-4, is a 160-kDa tyrosine kinase which phosphorylates cellular substrates when it is activated by the binding of insulin (1). IRS-4 was originally discovered in human embryonic kidney 293 cells. Subcellular fractionation revealed that about 50% of IRS-4 was located in cellular membranes, and immunofluorescence indicated that IRS-4 was concentrated at the plasma membrane. Immunoelectron microscopy conclusively established that a large portion of the IRS-4 was located at the cytoplasmic surface of the plasma membrane in both the unstimulated and insulin-treated states. IRS-4 was found to be associated with two src homology 2 (SH2) domain-containing proteins, phosphatidylinositol 3-kinase and Grb2, the adaptor to the guanine nucleotide exchange factor for Ras (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRS4
• Entrez:
  • 8471(Human)
  • 16370(Mouse)
  • 315350(Rat)
• Uniprot:
  • O14654(Human)