IRS1 [p Ser616] Antibody (738710)

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µg/mL Insulin for 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-IRS1 (S616) Monoclonal Antibody (Catalog # MAB39783) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-IRS1 (S616) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 738710
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IRS1 [p Ser616] Antibody (738710) Summary

• Immunogen: Phosphopeptide containing the human IRS1 S616 site
  Accession # P35568
• Modification: p Ser616
• Specificity: Detects human IRS1 when phosphorylated at S616.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRS1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRS1 [p Ser616] Antibody (738710)

• HIRS-1
• insulin receptor substrate 1
• IRS1
• IRS-1

Background

Human IRS1 (insulin receptor substrate 1) is a 150-180 kDa substrate that transmits signals from the insulin and IGF-I receptor to downstream signaling modulators. The 1242 amino acid (aa) human IRS1 contains a PH domain (aa 12-115), a PTB domain (aa 160-263) and ten PEST sequences (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. When PI-3 kinase and ERK1/2 are activated, insulin or angiotensin II can also stimulate phosphorylation on Ser616. The aa sequence surrounding pSer616 is identical between human, mouse and rat IRS-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRS1
• Uniprot:
  • P35568()