IRS1 Antibody (738710) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clone: 738710
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

IRS1 Antibody (738710) [Alexa Fluor® 350] Summary

• Immunogen: Phosphopeptide containing the human IRS1 S616 site
  Accession # P35568
• Specificity: Detects human IRS1 when phosphorylated at S616.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRS1 Antibody (738710) [Alexa Fluor® 350]

• HIRS-1
• insulin receptor substrate 1
• IRS1
• IRS-1

Background

Human IRS1 (insulin receptor substrate 1) is a 150-180 kDa substrate that transmits signals from the insulin and IGF-I receptor to downstream signaling modulators. The 1242 amino acid (aa) human IRS1 contains a PH domain (aa 12-115), a PTB domain (aa 160-263) and ten PEST sequences (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. When PI-3 kinase and ERK1/2 are activated, insulin or angiotensin II can also stimulate phosphorylation on Ser616. The aa sequence surrounding pSer616 is identical between human, mouse and rat IRS-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.