IRP2 Antibody

Product Discontinued

IRP2 Antibody Summary

• Immunogen: Peptide with sequence C-SIHYEGSEYKLSHGS corresponding to internal region according to NP_004127.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IREB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human liver lysates (calculated MW of 105 kDa band according to NP_004127.1). An additional band of unknown identity was also consistently observed at 60 kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient. IHC-P: Human kidney shows preferential staining in the cytoplasm of DCT.
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRP2 Antibody

• ACO3
• EC 4.2.1.3
• FLJ23381
• IRE-BP 2
• Iron regulatory protein 2
• iron-responsive element binding protein 2
• iron-responsive element-binding protein 2
• IRP2IRP2AD

Background

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. Hypoxia is important for normal tissue physiology as well as being a component of several pathophysiological conditions, including heart and cerebrovascular diseases and tumor growth. Because iron and oxygen are closely related, IRP2 also plays a role during hypoxia (low oxygen).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRP2 Antibody (NBP1-51966)(1)

Publication using NBP1-51966

• Wang W, Di X, D'Agostino RB et al. Excess capacity of the iron regulatory protein system. J Biol Chem 2007-08-01 [PMID: 17604281]

Bioinformatics

• Gene Symbol: IREB2
• Entrez:
  • 3658(Human)
  • 64602(Mouse)
  • 64831(Rat)
• Uniprot:
  • P48200(Human)