IRP2 Antibody

Images

 
Western Blot: IRP2 Antibody [NBP1-51966] - (0.3ug/ml) Analysis of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: IRP2 Antibody [NBP1-51966] - staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IRP2 Antibody Summary

Immunogen
Peptide with sequence C-SIHYEGSEYKLSHGS corresponding to internal region according to NP_004127.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IREB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 110 kDa band observed in human liver lysates (calculated MW of 105 kDa band according to NP_004127.1). An additional band of unknown identity was also consistently observed at 60 kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient. IHC-P: Human kidney shows preferential staining in the cytoplasm of DCT.
Theoretical MW
105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-51966.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRP2 Antibody

  • ACO3
  • EC 4.2.1.3
  • FLJ23381
  • IRE-BP 2
  • Iron regulatory protein 2
  • iron-responsive element binding protein 2
  • iron-responsive element-binding protein 2
  • IRP2IRP2AD

Background

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. Hypoxia is important for normal tissue physiology as well as being a component of several pathophysiological conditions, including heart and cerebrovascular diseases and tumor growth. Because iron and oxygen are closely related, IRP2 also plays a role during hypoxia (low oxygen).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87677
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB500-418
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
H00004891-M01
Species: Hu
Applications: WB, ELISA, IHC-P
NB200-585
Species: Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
NBP1-54918
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-84071
Species: Hu
Applications: WB, IHC, IHC-P
MAB7509
Species: Hu
Applications: WB, IHC
NBP1-87534
Species: Hu, Mu
Applications: IHC, IHC-P
NBP1-97507
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
H00003077-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-12553
Species: Hu
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-59068
Species: Hu
Applications: WB

Publications for IRP2 Antibody (NBP1-51966)(1)


Reviews for IRP2 Antibody (NBP1-51966) (0)

There are no reviews for IRP2 Antibody (NBP1-51966). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IRP2 Antibody (NBP1-51966) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional IRP2 Products

Bioinformatics Tool for IRP2 Antibody (NBP1-51966)

Discover related pathways, diseases and genes to IRP2 Antibody (NBP1-51966). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IRP2 Antibody (NBP1-51966)

Discover more about diseases related to IRP2 Antibody (NBP1-51966).
 

Pathways for IRP2 Antibody (NBP1-51966)

View related products by pathway.

Research Areas for IRP2 Antibody (NBP1-51966)

Find related products by research area.

Blogs on IRP2

There are no specific blogs for IRP2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IRP2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol IREB2
Entrez
Uniprot