IRF9 Antibody (1C10) - Azide and BSA Free

Immunohistochemistry-Paraffin: IRF9 Antibody (1C10) [H00010379-M01] - Analysis of monoclonal antibody to ISGF3G on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Sandwich ELISA: IRF9 Antibody (1C10) [H00010379-M01] - Detection limit for recombinant GST tagged ISGF3G is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IRF9 Antibody (1C10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse IRF9 Antibody (1C10) - Azide and BSA Free (H00010379-M01) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-IRF9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: ISGF3G (AAH35716, 1 a.a. ~ 393 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASGRARCTRKLRNWVVEQVESGQFPGVCWDDTAKTMFRIPWKHAGKQDFREDQDAAFFKAWAIFKGKYKEGDTGGPAVWKTRLRCALNKSSEFKEVPERGRMDVAEPYKVYQLLPPGIVSGQPGTQKVPSKRQHSSVSSERKEEEDAMQNCTLSPSVLQDSLNNEEEGASGGAVHSDIGSSSSSSSPEPQEVTDTTEAPFQGDQRSLEFLLPPEPDYSLLLTFIYNGRVVGEAQVQSLDCRLVAEPSGSESSMEQVLFPKPGPLEPTQRLLSQLERGILVASNPRGLFVQRLCPIPISWNAPQAPPGPGPHLLPSNECVELFRTAYFCRDLVRYFQGLGPPPKFQVTLNFWEESHGSSHTPQNLITVKMEQAFARYLLEQTPEQQAAILSLV
• Specificity: ISGF3G - interferon-stimulated transcription factor 3, gamma 48kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRF9 Antibody (1C10) - Azide and BSA Free

• IFN-alpha-responsive transcription factor subunit
• interferon regulatory factor 9
• Interferon-stimulated gene factor 3 gamma
• interferon-stimulated transcription factor 3, gamma (48kD)
• interferon-stimulated transcription factor 3, gamma 48kDa
• IRF9
• IRF-9
• ISGF3 p48 subunit
• ISGF3
• ISGF3G
• ISGF3GISGF-3 gamma
• p48
• Transcriptional regulator ISGF3 subunit gamma

Background

Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF9 Antibody (H00010379-M01)(1)

Publication using H00010379-M01

• Kyriakidis NC, Kapsogeorgou EK, Gourzi VC et al. TLR3 stimulation promotes Ro52/TRIM21 synthesis and nuclear redistribution in salivary gland epithelial cells, partially via type I interferon pathway. Clin Exp Immunol. 2014-08-07 [PMID: 25098814]

Bioinformatics

• Gene Symbol: IRF9
• Entrez:
  • 10379(Human)
• OMIM:
  • 147574(Human)
• Uniprot:
  • AAH35716(Human)