IRF2BP1 Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and MOLT-4 human acute lymphoblastic leukemia cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 hours. Gels were loaded with 30 µg of whole cell lysate (WCL) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRF2BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF2BP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IRF2BP1 was detected in immersion fixed 293T human embryonic kidney cell line using Sheep Anti-Human IRF2BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6800) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IRF2BP1 Antibody Summary

• Immunogen: E. coli-derived recombinant human IRF2BP1
  Ala188-Glu356
  Accession # Q8IU81
• Specificity: Detects human IRF2BP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IRF2BP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRF2BP1 Antibody

• DKFZP434M154
• interferon regulatory factor 2 binding protein 1
• interferon regulatory factor 2-binding protein 1
• IRF-2-binding protein 1
• IRF2BP1
• IRF-2BP1

Background

IRF2BP1 (Interferon regulatory factor 2 binding protein 1) is a 64-66 kDa member of the IFR2BP family of proteins. It is widely expressed, and known to bind to the C-terminus of IRF-2, creating a transcriptional repressor complex. IRF2BP1 also bind to JDP2 (Jun dimerization protein 2), promoting its ubiquitination. Human IRF2BP1 is 584 amino acids (aa) in length. It contains two Zn-finger domains (aa 6-67 and 498-584), one coiled-coil region (aa 197-217), a Cys-rich region (aa 503‑550), an N-terminal acetylation site at Ala2, and multiple utilized phosphorylation sites at Ser 66/125/186/384/421/436/453/457. Over aa 188-356, human and mouse IRF2BP1 are identical in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF2BP1
• Uniprot:
  • Q8IU81(Human)