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240-B) for 2 hours. Gels were loaded with 30 µg of whole cell lysate (WCL) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRF2BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF2BP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
Species: Hu Applications: WB, ICC/IF Host: Sheep Polyclonal
IRF2BP1 acts as a transcriptional repressor. Acts as a transcriptional corepressor in a IRF2-dependent manner. This repression is not mediated at least in part by histone deacetylase activities
