Intelectin-2 Antibody (450515) [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 450515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Intelectin-2 Antibody (450515) [Alexa Fluor® 488] Summary

• Immunogen: E.coli-derived recombinant human Intelectin-1
  Thr19-Ser298
  Accession # Q8WWA0
• Specificity: Detects human Intelectin-1 and human Intelectin-2 in direct ELISAs. Detects human, mouse, and rat Intelectin-1 and Intelectin-2 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-2 Antibody (450515) [Alexa Fluor® 488]

• HL-2
• intelectin 2
• Intelectin2
• Intelectin-2
• Intelectin-b
• Itln1b
• ITLN2
• Itlnb
• UNQ2789/PRO7179

Background

Intelectin-1 (intestinal lectin; also ITLN-1 and omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Intelectin-2 (ITLN-2; also endothelial lectin 2 and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells, and appears to participate in fungal microbe recognition. The human ITLN-2 precursor is 325 amino acids (aa) in length (SwissProt #:Q8WWU7). It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse ITLN-2 is reportedly GPI-linked, and mouse and human ITLN-2 show considerable C-terminal aa identity, it is not known if human ITNL-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 44-267). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 29-324, human ITLN-2 shares 79% aa sequence identity with mouse ITLN-2/ITLN-1b, and 85% aa sequence identity with human ITLN-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.