Intelectin-1/2 Antibody (450515) [Unconjugated]

Western blot shows lysates of recombinant human Intelectin-1 (Catalog # 9137-IN), recombinant human Intelectin-2, human small intestine tissue, mouse small intestine tissue, and rat small intestine tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Intelectin-1/2 Monoclonal Antibody (Catalog # MAB42542) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Intelectin-1/2 at approximately 36-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intelectin-1/2 was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human/Mouse Intelectin-1/2 Monoclonal Antibody (Catalog # MAB42542) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intelectin-1/2 was detected in immersion fixed paraffin-embedded sections of human ileum air-liquid interface using Mouse Anti-Human/Mouse Intelectin-1/2 Monoclonal Antibody (Catalog # MAB42542) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to mucus granules in goblet cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 450515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Intelectin-1/2 Antibody (450515) [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human Intelectin-1
  Thr19-Ser298
  Accession # Q8WWA0
• Specificity: Detects human Intelectin-1 and human Intelectin-2 in direct ELISAs. Detects human, mouse, and rat Intelectin-1 and Intelectin-2 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.5-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-1/2 Antibody (450515) [Unconjugated]

• Intelectin-1 / Intelectin-2
• Intelectin-1/2

Background

Intelectin-1 (intestinal lectin; also ITLN-1 and omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Intelectin-2 (ITLN-2; also endothelial lectin 2 and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by intestinal Paneth cells, and appears to participate in fungal microbe recognition. The human ITLN-2 precursor is 325 amino acids (aa) in length (SwissProt #:Q8WWU7). It contains a 26 aa signal sequence and a 299 aa mature region. Although mouse ITLN-2 is reportedly GPI-linked, and mouse and human ITLN-2 show considerable C-terminal aa identity, it is not known if human ITNL-2 is also GPI-linked. There is one fibrinogen-related domain in the mature molecule (aa 44-267). One potential splice variant is reported that shows a premature truncation after Gly264. Over aa 29-324, human ITLN-2 shares 79% aa sequence identity with mouse ITLN-2/ITLN-1b, and 85% aa sequence identity with human ITLN-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8WWA0()